Ciejak Magdalena, Olszewska Maria, Jakubowska Katarzyna, Zebiełowicz Dariusz, Safranow Krzysztof, Chlubek Dariusz
Zakład Chirurgii Stomatologicznej Pomorskiej Akademii Medycznej w Szczecinie, Szczecin.
Ann Acad Med Stetin. 2007;53(2):42-5.
One of the hypothetical reasons of the increased incidence of caries in women during the pregnancy may be the increased activity of alpha-amylase, which can be found in their saliva. The enzyme takes part in the process of decomposition of simple sugars, which make basic substrate for caries-causing bacteria. The aim of the paper was the evaluation of the influence of pregnancy and gestational age on the activity of alpha-amylase and the concentration of protein in women's saliva.
The examined group consisted of 64 pregnant women at age 17-39, between 21st and 40th week of pregnancy. The control group consisted of 44 healthy women at age 20-35, who were not pregnant. In saliva, which was taken before morning meal, without stimulation, protein concentration was determined by Bradford method and the activity of amylase was determined by kinetic method.
The activity of amylase correlated strongly and positively with protein concentration in saliva of both the pregnant (RS = +0.65; p < 0.00001) and the control group (RS = +0.74; p < 0.00001) women. There were no significant differences between examined parameters in the examined and the control group. It has been observed in the examined group, that there is the significant negative correlation between protein concentration in saliva and the week of pregnancy (RS = -0.35; p <0.01). It has been observed, in conducted researches, that there is no relation between the activity of amylase and the pregnancy and gestational age, which proves against the essential role of this enzyme in the increased caries incidence of pregnant women. However, the observed changes of total protein concentration in saliva during pregnancy, suggest that the exact cognition of proteins in pregnant women's saliva may reveal new mechanisms, which lead to an increase of caries risk.
孕期女性龋齿发病率增加的一个假设原因可能是其唾液中α-淀粉酶活性增强。该酶参与单糖的分解过程，而单糖是致龋菌的主要底物。本文旨在评估妊娠及孕周对女性唾液中α-淀粉酶活性和蛋白质浓度的影响。
研究组由64名年龄在17 - 39岁、孕期在第21至40周的孕妇组成。对照组由44名年龄在20 - 35岁、未怀孕的健康女性组成。在无刺激的情况下，于早餐前采集唾液，采用Bradford法测定蛋白质浓度，采用动力学方法测定淀粉酶活性。
孕妇组（RS = +0.65；p < 0.00001）和对照组女性（RS = +0.74；p < 0.00001）唾液中淀粉酶活性与蛋白质浓度均呈强正相关。研究组和对照组的检测参数之间无显著差异。在研究组中观察到，唾液中蛋白质浓度与孕周呈显著负相关（RS = -0.35；p <0.01）。在进行的研究中观察到，淀粉酶活性与妊娠及孕周无关，这证明该酶在孕妇龋齿发病率增加中不发挥关键作用。然而，孕期唾液中总蛋白浓度的观察变化表明，准确认识孕妇唾液中的蛋白质可能揭示导致龋齿风险增加的新机制。
