采用标准化方法采集的生理活跃人群唾液分析物的参考区间。
Reference intervals for saliva analytes collected by a standardized method in a physically active population.
机构信息
Laboratory of Exercise Biochemistry - LABEX, Biochemistry Department, Biology Institute, State University of Campinas, Cidade Universitária Zeferino Vaz, s/n, Barão Geraldo, CEP: 13083-970, CP6109, Campinas, SP, Brazil.
出版信息
Clin Biochem. 2011 Dec;44(17-18):1440-4. doi: 10.1016/j.clinbiochem.2011.09.012. Epub 2011 Oct 2.
OBJECTIVES
Our aims were to test a liquid-based saliva collection system for total antioxidant status (TAS), uric acid (UA), total protein concentration (TP) and salivary alpha-amylase (SAA) activity; to determine if these analytes in serum and saliva are correlated in a physically active population and to establish reference intervals for these parameters.
DESIGN AND METHODS
Participants in this study were 115 physically active males (18-20 years old). Saliva samples were collected using the Saliva Collection System (Greiner Bio-One) immediately before obtaining blood. Biochemical analyses were conducted using an Autolab Boehringer analyzer.
RESULTS
We found a correlation between UA and TP concentrations in serum and saliva samples. The reference intervals for TP and SAA activity in the morning were lower than in the afternoon (p<0.0001). The reference intervals for UA and TAS did not vary with the time of collection.
CONCLUSION
The establishment of reference intervals for these saliva constituents increases their diagnostic utility and allows for detection of physiological or pathological states.
目的
本研究旨在测试一种基于液体的唾液采集系统，用于检测总抗氧化状态（TAS）、尿酸（UA）、总蛋白浓度（TP）和唾液α-淀粉酶（SAA）活性；并确定在活跃人群中，血清和唾液中的这些分析物是否存在相关性，以及确定这些参数的参考区间。
设计和方法
本研究的参与者为 115 名活跃的男性（18-20 岁）。使用 Saliva Collection System（Greiner Bio-One）在采血前立即采集唾液样本。使用 Autolab Boehringer 分析仪进行生化分析。
结果
我们发现血清和唾液样本中 UA 和 TP 浓度之间存在相关性。TP 和 SAA 活性的清晨参考区间低于下午（p<0.0001）。UA 和 TAS 的参考区间不受采集时间的影响。
结论
这些唾液成分的参考区间的建立增加了它们的诊断效用，并允许检测生理或病理状态。
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