Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA.
Vet Microbiol. 2010 May 19;142(3-4):394-400. doi: 10.1016/j.vetmic.2009.11.002. Epub 2009 Nov 10.
An association between mucosa-adherent commensal bacteria and inflammatory bowel disease (IBD) has been proposed for humans. There are no reports characterizing the mucosa-adherent duodenal microbiota in dogs with idiopathic IBD using molecular methods. The aim of this study was to investigate differences in the mucosa-adherent duodenal microbiota between dogs with idiopathic IBD and healthy dogs. Duodenal biopsy samples were collected from seven dogs with IBD and seven healthy control dogs. DNA was extracted, 16S ribosomal RNA genes were amplified and 16S rRNA gene clone libraries were constructed and compared between groups. A total of 1035 clones were selected, and based on a 98% similarity criterion, 133 unique phylotypes were identified across all dogs. These phylotypes belonged to seven bacterial phyla: Proteobacteria (52.9%), Firmicutes (26.1%), Bacteroidetes (7.7%), Actinobacteria (8.6%), Fusobacteria (4.4%), Tenericutes (0.2%) and Verrucomicrobia (0.1%). Significant differences were identified in the relative abundance of several bacterial groups between dogs with IBD and healthy dogs (p<0.001). Healthy dogs and dogs with IBD clustered according to their disease status. Dogs with IBD had a significantly higher abundance of clones belonging to Alpha-, Beta-, and Gamma-proteobacteria (p<0.0001 for all classes), and a significantly lower abundance of Clostridia (p<0.0001). Bacteria of the genera Pseudomonas, Acinetobacter, Conchiformibious, Achromobacter, Brucella, and Brevundimonas, were significantly more abundant in dogs with IBD. In conclusion, significant differences of the mucosa-adherent duodenal microbiota were observed between dogs with idiopathic IBD and healthy dogs in this study. These results warrant further investigations into the role of the intestinal microbiota in the pathophysiology of canine IBD.
人们提出，黏膜定植的共生菌与炎症性肠病（IBD）之间存在关联。目前尚无使用分子方法描述特发性 IBD 犬黏膜定植十二指肠微生物群的报道。本研究旨在通过分子方法调查特发性 IBD 犬与健康犬之间黏膜定植十二指肠微生物群的差异。从 7 只 IBD 犬和 7 只健康对照犬中采集十二指肠活检样本。提取 DNA，扩增 16S 核糖体 RNA 基因，并构建和比较两组之间的 16S rRNA 基因克隆文库。共选择了 1035 个克隆，基于 98%相似性标准，在所有犬中鉴定出 133 个独特的菌型。这些菌型属于七个细菌门：变形菌门（52.9%）、厚壁菌门（26.1%）、拟杆菌门（7.7%）、放线菌门（8.6%）、梭杆菌门（4.4%）、柔膜菌门（0.2%）和疣微菌门（0.1%）。IBD 犬与健康犬之间的几个细菌群的相对丰度存在显著差异（p<0.001）。健康犬和 IBD 犬根据疾病状态聚类。IBD 犬中属于α-、β-和γ-变形菌门的克隆丰度显著增加（所有分类群 p<0.0001），而梭菌的丰度显著降低（p<0.0001）。假单胞菌属、不动杆菌属、拟杆菌属、无色杆菌属、布鲁氏菌属和短小杆菌属的细菌在 IBD 犬中明显更为丰富。总之，本研究观察到特发性 IBD 犬与健康犬之间黏膜定植十二指肠微生物群存在显著差异。这些结果表明有必要进一步研究肠道微生物群在犬 IBD 病理生理学中的作用。
