Lee Si-Young, Kinoshita Hiroshi, Ihara Fumio, Igarashi Yasuhiro, Nihira Takuya
International Center for Biotechnology, Osaka University, Suita, Osaka, Japan.
J Biosci Bioeng. 2008 May;105(5):476-80. doi: 10.1263/jbb.105.476.
Entomopathogenic fungi are unique owing to their versatile ability to produce many bioactive compounds and from the dependence of their morphological differentiation on the presence of insect-derived materials. An entomopathogenic fungus, Metarhizium anisopliae HF293, was found to show insect-material-dependent production of antibacterial compounds, which were purified to homogeneity from 10-d culture broth when the production reached maximum. Two compounds were isolated: the major compound was determined to be helvolic acid and the minor one was a novel derivative of helvolic acid (1,2-dihydrohelvolic acid). Discovery of a novel bioactive compound indicated that insect-derived material would be a useful factor for enhancing the diversity of compounds produced by entomopathogenic fungi.
昆虫病原真菌具有独特性，这归因于它们产生多种生物活性化合物的多样能力以及其形态分化对昆虫衍生材料存在的依赖性。发现一种昆虫病原真菌——金龟子绿僵菌HF293，其抗菌化合物的产生依赖于昆虫材料，当产量达到最大值时，从10天的培养液中纯化得到了纯度均一的抗菌化合物。分离出了两种化合物：主要化合物被确定为赫沃酸，次要化合物是赫沃酸的一种新型衍生物（1,2-二氢赫沃酸）。一种新型生物活性化合物的发现表明，昆虫衍生材料将是增强昆虫病原真菌产生的化合物多样性的一个有用因素。
