体外通过移植物抗宿主反应和混合淋巴细胞反应激活白血病病毒
Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro.
作者信息
Hirsch M S, Phillips S M, Solnik C, Black P H, Schwartz R S, Carpenter C B
出版信息
Proc Natl Acad Sci U S A. 1972 May;69(5):1069-72. doi: 10.1073/pnas.69.5.1069.
Spleen cells from BALB/c or CAF(1) mice released little or no detectable leukemia virus when cultured 2-7 days in vitro. In contrast, spleen cells of CAF(1) mice previously inoculated with parental BALB/c spleen cells released leukemia viruses in 10 of 11 cases studied. Cultures of a mixture of spleen cells from normal BALB/c and CAF(1) mice also contained leukemia viruses. Phytohemagglutinin induced the transformation of lymphocytes in cultures of CAF(1) or BALB/c spleen cells, but this transformation did not activate leukemia viruses. It is concluded that mixed lymphocyte cultures in vitro, just as graft-versus-host reactions in vivo, can activate leukemia viruses that are normally present in a repressed form. This activation is not solely a function of lymphocyte transformation. The activated mouse leukemia virus may subsequently account for the observed high incidence of neoplasia in graft-versus-host disease.
将BALB/c或CAF(1)小鼠的脾细胞在体外培养2 - 7天时，几乎不释放或检测不到白血病病毒。相比之下，先前接种了亲代BALB/c脾细胞的CAF(1)小鼠的脾细胞，在所研究的11个案例中有10个释放了白血病病毒。正常BALB/c和CAF(1)小鼠脾细胞混合物的培养物中也含有白血病病毒。植物血凝素可诱导CAF(1)或BALB/c脾细胞培养物中的淋巴细胞转化，但这种转化并未激活白血病病毒。得出的结论是，体外混合淋巴细胞培养，就如同体内的移植物抗宿主反应一样，能够激活通常以受抑制形式存在的白血病病毒。这种激活并非仅仅是淋巴细胞转化的作用。激活的小鼠白血病病毒随后可能是移植物抗宿主病中观察到的高肿瘤发生率的原因。
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