Ruddle N H, Conta B S, Leinwand L, Kozak C, Ruddle F, Besmer P, Baltimore D
J Exp Med. 1978 Aug 1;148(2):451-65. doi: 10.1084/jem.148.2.451.
The gene for the receptor for ecotropic murine leukemia virus (Rev) has been assigned to mouse chromosome 5. This determination was made possible by an analysis of somatic cell hybrids between mouse and Chinese hamster cells. The parents of these hybrids were A/HeJ or Mus poschiavinus peritoneal exudate cells or BALB/c primary embryo fibroblasts and E36, a Chinese hamster lung fibroblast deficient in hypoxanthine guanine phosphoribosyltransferase. Segregation of mouse chromosomes in these hybrids was analyzed by chromosome banding and isozyme expression. Cells were tested for their ability to absorb and replicate vesicular stomatitis virus (murine leukemia virus [MuLV]) pseudotype particles and ecotropic MuLV as measured by the XC test. The presence of chromosome 5 was essential for receptor expression as determined by three statistical procedures. Segregation of the receptor for ecotropic murine leukemia virus was also followed in two series of subclones. In both, receptor expression was syntenic with phosphoglucomutase-1, an isozyme which has been mapped to mouse chromosome 5.
嗜亲性鼠白血病病毒(Rev)受体的基因已被定位到小鼠的5号染色体上。这一判定是通过对小鼠细胞与中国仓鼠细胞之间的体细胞杂种进行分析得以实现的。这些杂种的亲本是A/HeJ或波氏小家鼠腹膜渗出细胞或BALB/c原代胚胎成纤维细胞,以及E36,一种次黄嘌呤鸟嘌呤磷酸核糖转移酶缺陷的中国仓鼠肺成纤维细胞。通过染色体显带和同工酶表达分析了这些杂种中小鼠染色体的分离情况。通过XC试验检测细胞吸收和复制水泡性口炎病毒(鼠白血病病毒[MuLV])假型颗粒和嗜亲性MuLV的能力。通过三种统计方法确定,5号染色体的存在对于受体表达至关重要。在两个系列的亚克隆中也追踪了嗜亲性鼠白血病病毒受体的分离情况。在这两个系列中,受体表达都与磷酸葡萄糖变位酶-1同线,磷酸葡萄糖变位酶-1是一种已被定位到小鼠5号染色体上的同工酶。