Nadagir Shobha D, Chunchanur Sneha K, Halesh L H, Yasmeen K, Chandrasekhar M R, Patil B S
Karnataka Institute of Medical Sciences, Hubli, India.
Southeast Asian J Trop Med Public Health. 2008 May;39(3):492-5.
Oropharyngeal candidiasis (OPC) continues to be a common opportunistic infection in patients infected with Human Immunodeficiency Virus (HIV) and is predictive of increasing immunosuppression. Though Candida albicans remains the predominant isolate, a rise in the frequency of isolation of non-albicans Candida (NAC) species is being observed. The levels of virulence and the sensitivities to available antifungal drugs vary among these species. Of 340 HIV seropositive patients in this study, 132 (38.8%) had oral lesions suggestive of candidiasis. Samples were collected from the lesion using sterile cotton swabs. Isolation and speciation were done by standard techniques. Antifungal drug susceptibility testing was done by macro broth dilution. The total number of Candida isolates was 135, of which, 45 (33.3%) were NAC species and 90 were C.albicans (66.6%). Of the NAC species, C. dubliniensis was the predominant pathogen (22,48.9%). Antifungal susceptibility testing showed that 14 (31.1%) of the NAC species and 11 (12.2%) of C. albicans were resistant to fluconazole (MIC > 8 microg/ml). A very high MIC of > 32 microg/ml was noted among the NAC species resistant to fluconazole.
口腔念珠菌病（OPC）仍然是感染人类免疫缺陷病毒（HIV）患者中常见的机会性感染，并且是免疫抑制加剧的预测指标。尽管白色念珠菌仍然是主要分离菌株，但非白色念珠菌（NAC）物种的分离频率正在上升。这些物种的毒力水平和对现有抗真菌药物的敏感性各不相同。在本研究的340名HIV血清阳性患者中，132名（38.8%）有提示念珠菌病的口腔病变。使用无菌棉签从病变处采集样本。通过标准技术进行分离和鉴定。通过微量肉汤稀释法进行抗真菌药物敏感性测试。念珠菌分离株总数为135株，其中45株（33.3%）为NAC物种，90株为白色念珠菌（66.6%）。在NAC物种中，都柏林念珠菌是主要病原体（22株，占48.9%）。抗真菌药敏试验表明，14株（31.1%）NAC物种和11株（12.2%）白色念珠菌对氟康唑耐药（MIC>8μg/ml）。在对氟康唑耐药的NAC物种中，观察到非常高的MIC>32μg/ml。
