Dolinková M, Dostálová I, Lacinová Z, Michalský D, Haluzíková D, Mráz M, Kasalický M, Haluzík M
3rd Department of Medicine, 1st Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.
Mol Cell Endocrinol. 2008 Sep 10;291(1-2):63-70. doi: 10.1016/j.mce.2008.05.001. Epub 2008 May 9.
The aim of the present study was to evaluate the expression profile of genes potentially related to metabolic complications of obesity in the whole adipose tissue and isolated adipocytes from subcutaneous (SAT) and visceral adipose tissue (VAT) from 12 non-diabetic obese women and 12 lean women. Real-time polymerase chain reaction was used for expression analysis of 41 genes of interest and two housekeeping genes. We found increased expression of specific proinflammatory and adipogenic genes and reduced expression of specific lipogenic and insulin signaling pathway genes in obese relative to lean women with no preferable localization in SAT or VAT depot. The gene expression significantly differed between adipocytes and adipose tissue but both contributed to the proinflammatory profile in obesity. We conclude that both SAT and VAT exhibit alterations in the expression of specific genes possibly contributing to proinflammatory and insulin resistance state and consequently to metabolic complications of obesity.
本研究的目的是评估12名非糖尿病肥胖女性和12名瘦女性的全脂肪组织以及分离自皮下脂肪组织（SAT）和内脏脂肪组织（VAT）的脂肪细胞中，与肥胖代谢并发症潜在相关的基因表达谱。采用实时聚合酶链反应对41个感兴趣的基因和两个管家基因进行表达分析。我们发现，与瘦女性相比，肥胖女性中特定促炎基因和成脂基因的表达增加，特定脂肪生成基因和胰岛素信号通路基因的表达减少，且在SAT或VAT库中无优先定位。脂肪细胞和脂肪组织之间的基因表达存在显著差异，但两者都促成了肥胖中的促炎特征。我们得出结论，SAT和VAT均表现出特定基因表达的改变，这可能导致促炎和胰岛素抵抗状态，进而导致肥胖的代谢并发症。
