Wetzel T, Candresse T, Ravelonandro M, Delbos R P, Mazyad H, Aboul-Ata A E, Dunez J
Station de Pathologie Végétale, INRA, Villenave d'Ornon, France.
J Gen Virol. 1991 Jul;72 ( Pt 7):1741-6. doi: 10.1099/0022-1317-72-7-1741.
The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together as one large open reading frame (ORF) in a fashion similar to the canonical genomic organization of other potyviruses. The large ORF encoding the polyprotein is followed by a 217 nucleotide non-coding region and a poly(A) tail. However, whereas the three PPV strains previously sequenced show levels of identity in excess of 98%, PPV-El Amar shows levels of heterogeneity of 20% in the nucleotide sequence and 10% in the amino acid sequence, when compared with these previously sequenced strains. The N-terminal region of the capsid protein, postulated to be involved in the aphid transmission mechanism of the virus, was found to be the region which differed most between PPV-El Amar and the other strains.
测定了从埃及分离的一种广泛分化的、蚜虫传播的李痘马铃薯Y病毒株（PPV - El Amar）RNA 3'末端4773个核苷酸的核苷酸序列。测序区域覆盖圆柱状内含体（CI）基因的羧基末端、假定的6K蛋白、NIa蛋白酶、NIb RNA聚合酶和衣壳蛋白基因，它们以类似于其他马铃薯Y病毒典型基因组组织的方式连接在一起，形成一个大的开放阅读框（ORF）。编码多蛋白的大ORF后面是一个217个核苷酸的非编码区和一个聚腺苷酸尾巴。然而，与之前测序的三个PPV株相比，之前测序的三个PPV株显示出超过98%的同一性水平，而PPV - El Amar在核苷酸序列中的异质性水平为20%，在氨基酸序列中的异质性水平为10%。衣壳蛋白的N末端区域被认为参与病毒的蚜虫传播机制，发现该区域在PPV - El Amar和其他株之间差异最大。
