El-Enany Nahed
University of Mansoura, Faculty of Pharmacy, Department of Analytical Chemistry, 35516, Mansoura, Egypt.
J AOAC Int. 2008 May-Jun;91(3):542-50.
A rapid, simple, and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous determination of metoclopramide (MT) and pyridoxine (PY) in a binary mixture. The method is based on measurement of the native fluorescence of these drugs at delta lambda = 80 nm in methanol. The different experimental parameters affecting the native fluorescence of the drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges of 0.02-0.4 and 0.1-2 microg/mL for MT and PY, respectively. The limits of detection were 0.003 and 0.007 microg/mL and the limits of quantification were 0.008 and 0.02 microg/mL for MT and PY, respectively. The proposed method was successfully applied to the determination of MT and PY in synthetic mixtures and in commercial syrup. The results were in good agreement with those obtained with a reported method. The high sensitivity attained by the proposed method allowed the determination of MT in spiked and real human plasma samples. The mean percent recoveries of MT from spiked and real human plasma (n = 3) were 93.72 +/- 3.15 and 89.72 +/- 2.19 respectively.
已开发出一种快速、简单且高灵敏度的二阶导数同步荧光法，用于同时测定二元混合物中的甲氧氯普胺（MT）和吡哆醇（PY）。该方法基于在甲醇中Δλ = 80 nm处测量这些药物的天然荧光。仔细研究并优化了影响药物天然荧光的不同实验参数。MT和PY的荧光 - 浓度曲线在0.02 - 0.4和0.1 - 2μg/mL范围内分别呈线性。MT和PY的检测限分别为0.003和0.007μg/mL，定量限分别为0.008和0.02μg/mL。所提出的方法成功应用于合成混合物和市售糖浆中MT和PY的测定。结果与报道方法获得的结果高度一致。所提出方法实现的高灵敏度使得能够测定加标和实际人血浆样品中的MT。加标和实际人血浆（n = 3）中MT的平均回收率分别为93.72±3.15和89.72±2.19。
