El-Enany N, Belal F, El-Shabrawy Y, Rizk M
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt;
Int J Biomed Sci. 2009 Jun;5(2):136-45.
A rapid, simple and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixture of chlorzoxazone (CLZ) and ibuprofen (IP). The method is based upon measurement of the synchronous fluorescence intensity of these drugs at Δλ=60 nm in methanol. The different experimental parameters affecting the fluorescence of the two drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.2-4 μg/mL and 0.1-1.6 μg/mL for CLZ and IP, respectively with lower detection limits (LOD) of 0.028 and 8.3 × 10(-3) μg/mL and quantification limits (LOQ) of 0.086 and 0.03 μg/mL for CLZ and IP, respectively. The proposed method was successfully applied for the determination of the two compounds in synthetic mixtures and in commercial capsules. The high sensitivity attained by the proposed method allowed the determination of both drugs and real human plasma samples. The mean % recoveries in real human plasma (n=3) were 87.69 ± 6.15 and 92.57 ± 4.39 for each of CLZ and IP respectively.
已开发出一种快速、简单且高度灵敏的二阶导数同步荧光法，用于同时分析氯唑沙宗（CLZ）和布洛芬（IP）的二元混合物。该方法基于在甲醇中Δλ = 60 nm处测量这些药物的同步荧光强度。仔细研究并优化了影响这两种药物荧光的不同实验参数。CLZ和IP的荧光-浓度曲线在0.2 - 4 μg/mL和0.1 - 1.6 μg/mL范围内分别呈线性，CLZ和IP的检测限（LOD）分别为0.028和8.3×10⁻³ μg/mL，定量限（LOQ）分别为0.086和0.03 μg/mL。所提出的方法成功应用于合成混合物和市售胶囊中两种化合物的测定。所提出的方法获得的高灵敏度使得能够测定这两种药物以及实际人体血浆样品。CLZ和IP在实际人体血浆（n = 3）中的平均回收率分别为87.69 ± 6.15和92.57 ± 4.39。
