从蓝桉果实中分离得到的蓝桉醇的抗菌活性
Antimicrobial activity of globulol isolated from the fruits of Eucalyptus globulus Labill.
作者信息
Tan Manliang, Zhou Ligang, Huang Yongfu, Wang Ye, Hao Xiaojiang, Wang Jingguo
机构信息
College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
出版信息
Nat Prod Res. 2008 May 10;22(7):569-75. doi: 10.1080/14786410701592745.
An antimicrobial sesquiterpene was separated by bioassay-guided isolation from the petroleum ether fraction of the ethanol crude extract of Eucalyptus globulus Labill (Myrtaceae) fruits, and was identified as globulol by physicochemical properties and spectroscopic analysis. Mycelial growth method revealed that the median effective inhibitory concentration (IC50) values of globulol on Alternaria solani, Fusarium oxysporum f.sp. niverum, F. graminearum, Rhizoctonia solani and Venturia pirina were 47.1 microg mL(-1), 114.3 microg mL(-1), 53.4 microg mL(-1), 56.9 microg mL(-1), 32.1 microg mL(-1) and 21.8 microg mL(-1), respectively. MTT-colorimetric assay revealed that IC50 values of globulol on Xanthomonas vesicatoria and Bacillus subtilis were 158.0 microg mL(-1) and 737.2 microg mL(-1), respectively. The results indicated that globulol could be a main antimicrobial compound in the ethanol crude extract of E. globulus fruits.
通过生物活性导向分离法从蓝桉（桃金娘科）果实乙醇粗提物的石油醚馏分中分离得到一种抗菌倍半萜，并通过理化性质和光谱分析鉴定为蓝桉醇。菌丝生长法表明，蓝桉醇对番茄早疫病菌、尖孢镰刀菌西瓜专化型、禾谷镰刀菌、立枯丝核菌和梨黑星病菌的半数有效抑制浓度（IC50）值分别为47.1μg mL-1、114.3μg mL-1、53.4μg mL-1、56.9μg mL-1、32.1μg mL-1和21.8μg mL-1。MTT比色法表明，蓝桉醇对番茄疮痂病菌和枯草芽孢杆菌的IC50值分别为158.0μg mL-1和737.2μg mL-1。结果表明，蓝桉醇可能是蓝桉果实乙醇粗提物中的主要抗菌化合物。
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