Vrchovská V, Spilková J, Valentão P, Sousa C, Andrade P B, Seabra R M
Faculty of Pharmacy, Department of Pharmacognosy, Charles University, Hradec Králové, Czech Republic.
Nat Prod Res. 2008 Jun 15;22(9):735-46. doi: 10.1080/14786410601132360.
The ability of Linaria vulgaris (Scrophulariaceae) infusion to act as a scavenger of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, reactive oxygen species (superoxide radical, hydroxyl radical, hypochlorous acid (HOCl)) and nitric oxide was investigated. The obtained data indicate that the infusion has a good scavenging activity against superoxide radical and is a very potent nitric oxide and DPPH scavenger. In hydroxyl radical assay a pro-oxidant capacity was noticed, especially for concentrations higher than 31.25 microg mL(-1). No effect was found against HOCl. A phytochemical study of this extract was also performed. The HPLC/UV analysis allowed the identification and quantification of eight organic acids (oxalic, aconitic, citric, ketoglutaric, ascorbic, malic, shikimic and fumaric acids). The phenolic composition of the lyophilised infusion was also determined by HPLC/DAD and four compounds were quantified, but, despite its high content, only linarin was managed to be identified.
研究了柳穿鱼（玄参科）浸剂作为2,2-二苯基-1-苦基肼自由基（DPPH）、活性氧（超氧阴离子自由基、羟基自由基、次氯酸（HOCl））和一氧化氮清除剂的能力。所得数据表明，该浸剂对超氧阴离子自由基具有良好的清除活性，并且是一种非常有效的一氧化氮和DPPH清除剂。在羟基自由基测定中，观察到了促氧化能力，尤其是对于浓度高于31.25μg mL⁻¹的情况。未发现对HOCl有影响。还对该提取物进行了植物化学研究。HPLC/UV分析使得能够鉴定和定量8种有机酸（草酸、乌头酸、柠檬酸、酮戊二酸、抗坏血酸、苹果酸、莽草酸和富马酸）。冻干浸剂的酚类成分也通过HPLC/DAD进行了测定，并且定量了4种化合物，但是，尽管其含量很高，仅鉴定出了木犀草苷。
