Kakar Maria, Amin Muhammad Usman, Alghamdi Saad, Sahibzada Muhammad Umar Khayam, Ahmad Nisar, Ullah Naseem
Department of Pharmacy, Abasyn University, Peshawar, KPK 25000, Pakistan.
Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, P.O. Box 715, Makkah 21955, Saudi Arabia.
Evid Based Complement Alternat Med. 2020 Mar 8;2020:3903682. doi: 10.1155/2020/3903682. eCollection 2020.
belongs to the Scrophulariaceae family and various plants of this family are known for their biological activities. The present study was focused on the isolation of bioactive compounds including a novel flavone 6,7,4'-trimethyl flavone (TMF) along with three known flavonoids such as quercetin, rutin, and a steroid -sitosterol which were isolated from the ethanolic extract of (Himalayan Wulfenia) through column chromatography and purified by using HPLC. Their structures were identified and elucidated through electron ionization mass spectroscopy (EIMS), 1DNMR (H-NMR and C-NMR), and 2DNMR (COSY, HMQC, and HMBC) spectroscopy. The antimicrobial activities of this novel compound were evaluated through agar well diffusion method, while antioxidant and cytotoxic activities were assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay and brine shrimp lethality assay, respectively. The NMR data revealed that TMF is a novel compound. TMF showed potential antibacterial and antifungal activities against (MIC = 128 g/ml) and (MIC = 128 g/ml). The cytotoxic potential of TMF was determined from brine shrimp lethality assay with LD of 127.01 g/ml. The free-radical scavenging potential of TMF at various concentrations implicated its strong antioxidant activity in vitro. The results revealed that TMF demonstrated substantial antimicrobial activity against and , strong antioxidant activity, and moderately cytotoxic activity.
属于玄参科，该科的多种植物以其生物活性而闻名。本研究聚焦于生物活性化合物的分离，包括一种新型黄酮6,7,4'-三甲基黄酮（TMF）以及三种已知黄酮类化合物，如槲皮素、芦丁，还有一种甾体——β-谷甾醇，它们是通过柱色谱从（喜马拉雅狼紫草）的乙醇提取物中分离出来的，并通过高效液相色谱法进行纯化。通过电子电离质谱（EIMS）、一维核磁共振（氢核磁共振和碳核磁共振）以及二维核磁共振（COSY、HMQC和HMBC）光谱对它们的结构进行了鉴定和阐明。通过琼脂孔扩散法评估了这种新型化合物的抗菌活性，而抗氧化和细胞毒性活性分别通过2,2-二苯基-1-苦基肼（DPPH）自由基清除试验和卤虫致死试验进行评估。核磁共振数据表明TMF是一种新型化合物。TMF对（最低抑菌浓度=128μg/ml）和（最低抑菌浓度=128μg/ml）显示出潜在的抗菌和抗真菌活性。TMF的细胞毒性潜力通过卤虫致死试验确定，半数致死剂量为127.01μg/ml。TMF在不同浓度下的自由基清除潜力表明其在体外具有很强的抗氧化活性。结果表明，TMF对和表现出显著的抗菌活性、很强的抗氧化活性以及中等程度的细胞毒性活性。
