Mareck Ute, Geyer Hans, Opfermann Georg, Thevis Mario, Schänzer Wilhelm
Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.
J Mass Spectrom. 2008 Jul;43(7):877-91. doi: 10.1002/jms.1457.
Steroid profiling is one of the most versatile and informative screening tools for the detection of steroid abuse in sports drug testing. Concentrations and ratios of various endogenously produced steroidal hormones, their precursors and metabolites including testosterone (T), epitestosterone (E), dihydrotestosterone (DHT), androsterone (And), etiocholanolone (Etio), dehydroepiandrosterone (DHEA), 5alpha-androstane-3alpha,17beta-diol (Adiol), and 5beta-androstane-3alpha,17beta-diol (Bdiol) as well as androstenedione, 6alpha-OH-androstenedione, 5beta-androstane-3alpha,17alpha-diol (17-epi-Bdiol), 5alpha-androstane-3alpha,17alpha-diol (17-epi-Adiol), 3alpha,5-cyclo-5alpha-androstan-6beta-ol-17-one (3alpha,5-cyclo), 5alpha-androstanedione (Adion), and 5beta-androstanedione (Bdion) add up to a steroid profile that is highly sensitive to applications of endogenous as well as synthetic anabolic steroids, masking agents, and bacterial activity. Hence, the knowledge of factors that do influence the steroid profile pattern is a central aspect, and pharmaceutical (application of endogenous steroids and various pharmaceutical preparations), technical (hydrolysis, derivatization, matrix), and biological (bacterial activities, enzyme side activities) issues are reviewed.
类固醇分析是体育药物检测中用于检测类固醇滥用的最通用且信息丰富的筛查工具之一。各种内源性产生的甾体激素、其前体和代谢物的浓度及比例，包括睾酮（T）、表睾酮（E）、双氢睾酮（DHT）、雄酮（And）、本胆烷醇酮（Etio）、脱氢表雄酮（DHEA）、5α-雄甾烷-3α,17β-二醇（Adiol）和5β-雄甾烷-3α,17β-二醇（Bdiol），以及雄烯二酮、6α-羟基雄烯二酮、5β-雄甾烷-3α,17α-二醇（17-表-Bdiol）、5α-雄甾烷-3α,17α-二醇（17-表-Adiol）、3α,5-环-5α-雄甾烷-6β-醇-17-酮（3α,5-环）、5α-雄甾烷二酮（Adion）和5β-雄甾烷二酮（Bdion），共同构成了一种对内源性及合成合成代谢类固醇、掩蔽剂和细菌活性的应用高度敏感的类固醇谱。因此，了解影响类固醇谱模式的因素是一个核心方面，本文将对药物（内源性类固醇和各种药物制剂的应用）、技术（水解、衍生化、基质）和生物学（细菌活性、酶的副活性）问题进行综述。
