用于研究GroEL单个分子构象动力学的光开关设计。
Design of an optical switch for studying conformational dynamics in individual molecules of GroEL.
作者信息
Frank Gabriel A, Kipnis Yakov, Smolensky Elena, Daube Shirley S, Horovitz Amnon, Haran Gilad
机构信息
Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
出版信息
Bioconjug Chem. 2008 Jul;19(7):1339-41. doi: 10.1021/bc800118j. Epub 2008 Jun 24.
Abstract
We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules.
摘要
我们描述了伴侣蛋白GroEL中一种光开关的设计，该光开关通过其ATP和共伴侣蛋白GroES驱动的构象变化来打开和关闭。这种基于荧光团和猝灭剂的开关被设计到伴侣蛋白的单环变体中，并在蛋白质在其变构状态之间转变时，显示出荧光强度的显著调制。因此，它构成了一种灵敏的探针，可用于研究该分子机器在整体和单分子水平上变构转变的动力学。
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