University of Cambridge, Department of Plant Sciences, Downing Site, Cambridge, CB2 3EA, UK.
Plant Methods. 2008 Jun 23;4:15. doi: 10.1186/1746-4811-4-15.
Plant cell death is a normal process during plant development. Mutant plants may exhibit misregulation of this process, which can lead to severe growth defects. Simple ways of visualising cell death in living plant tissues can aid the study of plant development and physiology.
Spectral variants of the fluorescent SYTOX dyes were tested for their usefulness for the detection of non-viable cells within plant embryos and roots using confocal laser-scanning microscopy. The dyes were selective for non-viable cells and showed very little background staining in living cells. Simultaneous detection of SYTOX dye and fluorescent protein (e.g. GFP) fluorescence was possible.
The fluorescent SYTOX dyes are useful for an easy and quick first assay of plant cell viability in living plant samples using fluorescence and confocal laser-scanning microscopy.
植物细胞死亡是植物发育过程中的一个正常过程。突变植物可能表现出这种过程的失调，这可能导致严重的生长缺陷。在活体植物组织中可视化细胞死亡的简单方法可以帮助研究植物的发育和生理学。
使用共焦激光扫描显微镜测试了荧光 SYTOX 染料的光谱变体在检测植物胚胎和根内非存活细胞中的有用性。这些染料对非存活细胞具有选择性，并且在活细胞中几乎没有背景染色。可以同时检测 SYTOX 染料和荧光蛋白（例如 GFP）的荧光。
荧光 SYTOX 染料可用于使用荧光和共焦激光扫描显微镜快速简便地检测活体植物样品中植物细胞的活力。
