[中国HIV阳性个体对结核分枝杆菌蛋白ESAT-6的特异性T细胞应答]
[Specific T-cell responses to Mycobacterium tuberculosis protein ESAT-6 in Chinese HIV positive individuals].
作者信息
Zhang Bin, Lun Wen-hui, Cheng Jun, Zhao Long-feng, Li Xing-wang, Han Ning, Wang Qi, Liang Yun-lei, Mao Yu
机构信息
The First Affiliated Hospital, Shanxi Medical University, Taiyuan, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Apr;22(2):124-6.
OBJECTIVE
To detect TB specific T cell responses by using the recombinant ESAT-6 protein as stimulus in Chinese HIV infected patients.
METHODS
ELISPOT-IFN-gamma assay by using the recombinant ESAT-6 protein as stimulus to detect specific T cell responses in HIV+ patients with or without clinical manifestation of TB diseases.
RESULTS
Recombinant ESAT-6 protein specific T cell responses show significant high frequencies in both of TB patients with or without HIV infection than that in the healthy control and HIV+ group without clinical TB diseases.
CONCLUSION
The ELISPOT-IFN-gamma assay by using recombinant ESAT-6 protein as stimulus could be used in diagnoses of TB infection in Chinese HIV infected patients.
目的
在中国感染人类免疫缺陷病毒（HIV）的患者中，使用重组早期分泌性抗原靶6（ESAT-6）蛋白作为刺激物来检测结核特异性T细胞反应。
方法
采用以重组ESAT-6蛋白为刺激物的酶联免疫斑点法（ELISPOT）检测干扰素-γ，以检测有或无结核病临床表现的HIV阳性患者的特异性T细胞反应。
结果
无论是否感染HIV，结核病患者中重组ESAT-6蛋白特异性T细胞反应的频率均显著高于健康对照组和无临床结核病的HIV阳性组。
结论
以重组ESAT-6蛋白为刺激物的ELISPOT-干扰素-γ检测法可用于诊断中国HIV感染患者的结核感染。
Zotero 插件