Wu Yulin, Chen Haigang, Li Zhaoli, Sun Liwei, Qu Mengmeng, Li Mei, Kong Zhiming
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
J Environ Sci (China). 2008;20(4):487-91. doi: 10.1016/s1001-0742(08)62084-7.
The potential harm of organic pollutants in drinking water to human health is widely focused on in the world; more and more pollutants with genotoxic substances are released into the aquatic environment. Water source samples were collected from 7 different localities of Nanjing City. The potential genotoxicity of organic extracts from drinking water sources were investigated by means of the comet assay in human peripheral lymphocytes. The results showed that all the organic extracts from all the water source samples could induce DNA damages of human peripheral blood lymphocytes at different levels. A significant difference (P < 0.01) was observed when compared with the solvent control. The DNA damage increased with the increase of the dosage of the original water source. Significant differences of DNA damage were observed in different drinking water sources, as shown by the multiple comparisons analysis at the dosage of 100x; the degree of DNA damage treated by Hushu waterworks (at town level) was the most serious, the arbitrary units (AU) was 141.62 +/- 6.96, however, that of Shangyuanmen waterworks (at city level) was only 109.64 +/- 2.97. The analysis also revealed that the genotoxicity of town's water sources was higher than that of the city. The results demonstrated that the comet assay can be successfully applied to the genotoxicity monitoring programs of drinking water sources.
饮用水中有机污染物对人体健康的潜在危害在全球范围内受到广泛关注；越来越多含有遗传毒性物质的污染物被排放到水环境中。从南京市7个不同地点采集了水源样本。采用彗星试验法，以人外周血淋巴细胞为材料，对饮用水源有机提取物的潜在遗传毒性进行了研究。结果表明，所有水源样本的有机提取物均能不同程度地诱导人外周血淋巴细胞DNA损伤。与溶剂对照组相比，差异有统计学意义（P<0.01）。DNA损伤程度随原水剂量的增加而增加。在100倍剂量下进行多重比较分析，不同饮用水源的DNA损伤存在显著差异；湖熟水厂（镇级）处理的DNA损伤程度最严重，任意单位（AU）为141.62±6.96，而上元门水厂（市级）的DNA损伤程度仅为109.64±2.97。分析还表明，镇级水源的遗传毒性高于市级水源。结果表明，彗星试验可成功应用于饮用水源遗传毒性监测项目。
