Sentani Kazuhiro, Oue Naohide, Tashiro Takashi, Sakamoto Naoya, Nishisaka Takashi, Fukuhara Toshiyuki, Taniyama Kiyomi, Matsuura Hiroo, Arihiro Koji, Ochiai Atsushi, Yasui Wataru
Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Am J Surg Pathol. 2008 Aug;32(8):1182-9. doi: 10.1097/PAS.0b013e318163a8f8.
Signet-ring cell carcinoma (SRCC) is a unique subtype of adenocarcinoma that is characterized by abundant intracellular mucin accumulation and a crescent-shaped nucleus displaced toward one end of the cell. Identification of an SRCC's primary site is important for better planning of patient management because the treatment and prognosis differs markedly depending on the origin of the SRCC. In the present study, we analyzed the immunohistochemical characteristics of 94 cases of SRCC, including 21 cases of gastric SRCC, 16 of colorectal SRCC, 10 of breast SRCC, and 47 of pulmonary SRCC, with antibodies against Reg IV and claudin-18, which we previously identified as gastric cancer-related genes. We also tested known markers cytokeratin 7, cytokeratin 20, MUC2, MUC5AC, caudal-related homeobox gene 2 (CDX2), thyroid transcription factor-1, mammaglobin, gross cystic disease fluid protein 15, and estrogen receptor. All 21 cases of gastric SRCC and 16 cases of colorectal SRCC were positive for Reg IV, and the remaining SRCCs were negative. Eighteen of 21 (86%) gastric SRCCs and 6 of 16 (38%) colorectal SRCCs were positive for claudin-18, whereas another SRCCs were negative. In conclusion, Reg IV staining and claudin-18 staining can aid in diagnosis of gastrointestinal SRCC.
印戒细胞癌（SRCC）是腺癌的一种独特亚型，其特征是细胞内有大量粘蛋白积聚，细胞核呈新月形并向细胞一端移位。确定SRCC的原发部位对于更好地规划患者管理很重要，因为根据SRCC的起源不同，其治疗方法和预后有显著差异。在本研究中，我们用针对Reg IV和claudin-18的抗体分析了94例SRCC的免疫组化特征，这两种抗体我们之前鉴定为与胃癌相关的基因。我们还检测了已知标志物细胞角蛋白7、细胞角蛋白20、MUC2、MUC5AC、尾型相关同源盒基因2（CDX2）、甲状腺转录因子-1、乳腺珠蛋白、巨大囊肿病液体蛋白15和雌激素受体。所有21例胃SRCC和16例结直肠SRCC的Reg IV均呈阳性，其余SRCC为阴性。21例胃SRCC中有18例（86%）和16例结直肠SRCC中有6例（38%）的claudin-18呈阳性，而其他SRCC为阴性。总之，Reg IV染色和claudin-18染色有助于胃肠道SRCC的诊断。
