Kumar P K, Lonsane B K
Fermentation Technology and Bioengineering Discipline, Central Food Technological Research Institute, Mysore 570 013, India.
Biotechnol Bioeng. 1987 Aug 5;30(2):267-71. doi: 10.1002/bit.260300217.
Five strains each of Gibberella fujikuroi and Fusarium monoliforme were screened to select G. fujikuroi P-3, a strain capable of giving consistent production of gibberellic acid (GA(3)) by solid state fermentation (SSF). The comparative production of GA(3) by SSF and submerged fermentation (SmF) indicated better productivity with the former technique. The accumulation of GA(3) was 1.626 times higher in the case of SSF. On the basis of available carbohydrates in the media, the percent conversions were 0.096 and 0.156 in SmF and SSF, respectively. The use of coarse wheat bran of the particle size of 0.3-0.4 cm resulted in an increase of 2.5 times in the yield of GA(3). The enrichment of commercial wheat bran with soluble starch gave enhanced accumulation to an extent of 3.5 times. The relation between GA(3) production and cell growth in SSF was similar to that encountered in SmF. The consistent and improved yields to a tune of 1.22 g GA(3) per kilogram dry moldy bran (DMB) establish the potential and feasibility of SSF for the production of GA(3) by G. fujikuroi P-3. On preliminary cost analysis, a net savings of about 60% and 50% on fermentation medium cost and the expenditure on down-stream processing, respectively, as compared to the presently employed SmF technique was evident.
对藤仓赤霉（Gibberella fujikuroi）和串珠镰刀菌（Fusarium monoliforme）各5个菌株进行了筛选，以选出藤仓赤霉P - 3菌株，该菌株能够通过固态发酵（SSF）持续生产赤霉酸（GA(3)）。通过固态发酵和深层发酵（SmF）对GA(3)的产量进行比较，结果表明前一种技术具有更高的生产率。在固态发酵情况下，GA(3)的积累量高出1.626倍。基于培养基中可用的碳水化合物，深层发酵和固态发酵的转化率分别为0.096和0.156。使用粒径为0.3 - 0.4厘米的粗麦麸，GA(3)产量提高了2.5倍。用可溶性淀粉富集商业麦麸，GA(3)积累量提高到了3.5倍。固态发酵中GA(3)产量与细胞生长之间的关系与深层发酵中相似。藤仓赤霉P - 3通过固态发酵持续且提高的产量达到每千克干发霉麸皮（DMB）1.22克GA(3)，这证明了固态发酵生产GA(3)的潜力和可行性。初步成本分析表明，与目前采用的深层发酵技术相比，发酵培养基成本和下游加工支出分别净节省约60%和50%。
