Liang Lei, Zhang Xu-Hui, Zhou Yi, Huang Yu-Juan, Deng Hong-Zhu
College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jun;28(6):982-5.
To observe the protective effect of Oviductus Ranae (OR) capsules on the reproductive organs in an aged mouse model established by D-galactose injection.
Forty-eight female Kunming mice were randomly divided into 4 equal groups, namely the high- and low-dose OR groups, diethylstilbestrol (DT) group, and model group. The mice received subcutaneous injection of D-galactose for 6 weeks to establish aging models. Another 12 mice were injected daily with normal saline (NS) to serve as the normal control group. From the third week of the experiment, the mice were given oral OR at low or high doses (in the OR groups) or vegetable oil (in the model or control groups) till the sixth week. In the last two weeks, the vaginal smears were obtained from the mice for evaluating the changes of the vaginal keratinocytes and counting the days of estrus. After completion of drug administration, all the mice were sacrificed and the serum content of estradiol (E(2)) was detected by radioimmunoassay, with the ovarian and uterine indices determined. The ovarian and uterine pathologies were observed using HE staining, and SOD and MDA activities in the ovary and uterus were also assessed.
OR obviously increased E(2) level and the ovarian and uterine indices in the aged mice, also alleviating the pathological change of the ovary and uterus. OR substantially depressed MDA content and enhanced SOD activity in the ovary and uterus.
OR has definite antioxidative effects and ameliorates the degenerative changes of the reproductive organs in mouse models of aging.
观察林蛙油胶囊对注射D-半乳糖建立的衰老小鼠模型生殖器官的保护作用。
将48只雌性昆明小鼠随机分为4组，每组12只，即林蛙油高、低剂量组、己烯雌酚组和模型组。连续6周皮下注射D-半乳糖建立衰老模型，另取12只小鼠每日注射生理盐水作为正常对照组。从实验第3周起，林蛙油组小鼠分别灌胃低、高剂量林蛙油，模型组和对照组灌胃植物油，直至第6周。最后2周取小鼠阴道涂片，观察阴道角质化细胞变化并计算动情周期天数。给药结束后，处死所有小鼠，采用放射免疫法检测血清雌二醇（E₂）含量，测定卵巢和子宫指数。取卵巢和子宫组织进行HE染色观察病理变化，并检测卵巢和子宫组织中超氧化物歧化酶（SOD）和丙二醛（MDA）活性。
林蛙油可明显提高衰老小鼠血清E₂水平及卵巢和子宫指数，减轻卵巢和子宫的病理改变，显著降低卵巢和子宫组织中MDA含量，增强SOD活性。
林蛙油具有一定的抗氧化作用，可改善衰老小鼠模型生殖器官的退行性变化。
