Wu Min, Chen Yi-Zhong, Hu Zu-Qiong, Jiang Ling-Xiao, Chen Qing
Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jun;28(6):1061-3.
To investigate the presence of pathogenicity island (PAI)-associated genes in the enterococcal isolates.
Using PCR and hybridization methods, PAI-associated genes were detected in 155 enteococcal strains isolated from clinical patients and healthy individuals.
Among the 155 enterococcal isolates, 137 (88.39%) carried at least one of PAI-associated genes, namely hyd (positivity rate of 81.94%), psaA (78.06%), nuc (57.42%), esp (53.55%), cylB (52.90%), and gls24-like (38.06%) genes. Expect for esp gene, the other 5 genes showed higher positivity rates in the E. faecalis strains than in the E. faecium strains, and this difference was statistically significant for the genes nuc, cylB, and gls24-like. The positivity rates and the number of these genes in the E. faecalis from clinical isolates were both significantly higher than those in the strains isolated from healthy individuals.
The data show a wide distribution of the PAI-associated genes among the enterococcal strains, and E. faecalis strains are more likely than E. faecium strains to be positive for the 6 genes, which are present at significant higher rates in the clinically isolated samples than in that from healthy individuals.
调查肠球菌分离株中与致病岛（PAI）相关基因的存在情况。
采用聚合酶链反应（PCR）和杂交方法，对从临床患者和健康个体中分离出的155株肠球菌进行PAI相关基因检测。
在155株肠球菌分离株中，137株（88.39%）携带至少一种PAI相关基因，即hyd（阳性率81.94%）、psaA（78.06%）、nuc（57.42%）、esp（53.55%）、cylB（52.90%）和gls24样（38.06%）基因。除esp基因外，其他5种基因在粪肠球菌菌株中的阳性率高于屎肠球菌菌株，且nuc、cylB和gls24样基因的差异具有统计学意义。临床分离的粪肠球菌中这些基因的阳性率和数量均显著高于从健康个体中分离的菌株。
数据表明PAI相关基因在肠球菌菌株中广泛分布，粪肠球菌菌株比屎肠球菌菌株更易对这6种基因呈阳性，且这些基因在临床分离样本中的存在率显著高于健康个体样本。
