Spácil Zdenek, Nováková Lucie, Solich Petr
Department of Analytical Chemistry, Faculty of Pharmacy, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Talanta. 2008 Jun 30;76(1):189-99. doi: 10.1016/j.talanta.2008.02.021. Epub 2008 Mar 4.
Two novel chromatographic methods both based on utilization of sub-2-micron particle columns were developed for the analysis of phenolic compounds in this work. An HPLC system was equipped with C(18) silica-based analytical column (50 mm x 4.6 mm, 1.8 microm) and a UPLC system with ethylene-bridged hybrid C(18) analytical column (100 mm x 2.1 mm, 1.7 microm). In total 34 phenolic substances were divided into groups of phenolic acids, flavonoids, catechins and coumarins and were analysed in sequence using different gradient methods. System suitability test data, including repeatability of retention time and peak area, mean values of asymmetry factor, resolution, peak capacity and the height equivalent of a theoretical plate were determined for each gradient method by 10 replicate injections. The developed methods were applied in the analysis of real samples (grape wines, teas).
本研究开发了两种基于使用亚2微米颗粒柱的新型色谱方法，用于分析酚类化合物。一个高效液相色谱（HPLC）系统配备了C(18)硅胶基质分析柱（50 mm×4.6 mm，1.8微米），一个超高效液相色谱（UPLC）系统配备了乙烯桥联杂化C(18)分析柱（100 mm×2.1 mm，1.7微米）。总共34种酚类物质被分为酚酸、黄酮类、儿茶素和香豆素类，并使用不同的梯度方法依次进行分析。通过10次重复进样，为每种梯度方法测定了系统适用性测试数据，包括保留时间和峰面积的重复性、不对称因子的平均值、分离度、峰容量和理论塔板高度等效值。所开发的方法应用于实际样品（葡萄酒、茶叶）的分析。
