Biology Department, Science College, King Khalid University, Abha, Kingdom of Saudi Arabia.
Biotechnology Department, College of Science, Taif University, Taif, Kingdom of Saudi Arabia.
Environ Sci Pollut Res Int. 2019 Feb;26(6):5990-5999. doi: 10.1007/s11356-018-4011-x. Epub 2019 Jan 6.
The aims of this study were to determine the polyphone content of grape seed extract (GSE) and to assess their protective effects against zearalenone (ZEN)-induced maternal toxicity and in utero development defects in mice. Five groups of pregnant mice were treated orally during days 6-13 of gestation as follows: control group, corn oil as vehicle (0.1 ml/mice)-treated group, ZEN-treated group (25 mg/kg b.w), GSE-treated group (150 mg/kg b.w.), and ZEN plus GSE-treated group. All animals were sacrificed on the 19th day of gestation and samples of bone marrow were collected for the micronucleus assay. The maternal and developmental toxicity were carried out. The HPLC analyses revealed that GES is rich in gallic acid, syringic acid, vanillin, quercetin, and coumaric acid. ZEN administration resulted in severe maternal and developmental toxicity which included an increase of micronuclei formation in bone marrow, decreased maternal weight gain, and litter weight. It also induces fetal growth retardation, increased number of the aborted dams and resorbed fetuses, abnormality of fetal bone ossification, and number of fetuses with a hematoma. GSE showed positive effects on the pregnant mice and the developing fetuses. Moreover, it counteracted the detrimental effects of ZEN in dams and fetuses. It could be concluded that polyphenols in GSE are a promising candidate to protect against ZEN toxicity in highly endemic areas.
本研究旨在确定葡萄籽提取物（GSE）中的多酚含量，并评估其对玉米赤霉烯酮（ZEN）诱导的母体毒性和宫内发育缺陷的保护作用。将五组怀孕小鼠在妊娠第 6-13 天经口处理，具体如下：对照组，玉米油作为载体（0.1ml/只）处理组，ZEN 处理组（25mg/kg bw），GSE 处理组（150mg/kg bw），以及 ZEN+GSE 处理组。所有动物于妊娠第 19 天处死，采集骨髓样本进行微核试验。进行母体和发育毒性试验。HPLC 分析表明 GES 富含没食子酸、丁香酸、香草醛、槲皮素和香豆酸。ZEN 给药导致严重的母体和发育毒性，包括骨髓中微核形成增加、母体体重增加和窝重减少。它还会导致胎儿生长迟缓、流产母鼠和吸收胎儿数量增加、胎儿骨骼骨化异常以及胎儿血肿数量增加。GSE 对怀孕小鼠和发育中的胎儿均有积极作用。此外，它还能拮抗 ZEN 对母体和胎儿的有害影响。综上所述，GSE 中的多酚类化合物是一种很有前途的候选物，可以预防高度流行地区 ZEN 毒性。
