Sung Ho Joong, Kim Yoon Suk, Kang Hyereen, Ko Jesang
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Exp Mol Med. 2008 Jun 30;40(3):332-8. doi: 10.3858/emm.2008.40.3.332.
Chemokines and chemokine receptors play a role in migration of circulating leukocytes to the region of inflammation. Human LZIP is an uncharacterized transcription factor and is known to participate in leukotactin (Lkn)-1/CCL15-induced cell migration. We investigated the role of human LZIP in expression of CC chemokine receptors (CCRs) and its involvement in monocyte migration. RNase protection analysis showed that LZIP increased mRNA expression of CCR2 and CCR1 in THP-1 cells. Surface expressions of both CCR2 and CCR1 were also increased by LZIP. Results from an electrophoretic mobility shift assay showed that LZIP binds to the C/EBP element in the CCR2 promoter. LZIP also enhanced the chemotactic activities of monocyte chemoattractant protein-1/CCL2 and Lkn-1. These results suggest that LZIP regulates expression of chemokine receptors that are involved in monocyte migration.
趋化因子和趋化因子受体在循环白细胞向炎症区域的迁移中发挥作用。人LZIP是一种未被充分研究的转录因子，已知其参与白细胞趋化素（Lkn）-1/CCL15诱导的细胞迁移。我们研究了人LZIP在CC趋化因子受体（CCR）表达中的作用及其在单核细胞迁移中的参与情况。核糖核酸酶保护分析表明，LZIP增加了THP-1细胞中CCR2和CCR1的mRNA表达。LZIP还增加了CCR2和CCR1的表面表达。电泳迁移率变动分析结果表明，LZIP与CCR2启动子中的C/EBP元件结合。LZIP还增强了单核细胞趋化蛋白-1/CCL2和Lkn-1的趋化活性。这些结果表明，LZIP调节参与单核细胞迁移的趋化因子受体的表达。
