Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey.
Lab Chip. 2016 Jul 5;16(14):2572-95. doi: 10.1039/c6lc00521g.
This review targets diversified oligonucleotide-based biodetection techniques, focusing on the use of microresonators of whispering gallery mode (WGM) type as optical biosensors mostly integrated with lab-on-a-chip systems. On-chip and microfluidics combined devices along with optical microresonators provide rapid, robust, reproducible and multiplexed biodetection abilities in considerably small volumes. We present a detailed overview of the studies conducted so far, including biodetection of various oligonucleotide biomarkers as well as deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) and proteins. We particularly advert to chemical surface modifications for specific and selective biosensing.
本综述针对多元化的基于寡核苷酸的生物检测技术，重点介绍了 whispering gallery mode (WGM) 型微谐振器作为光学生物传感器的应用，这些传感器主要与芯片实验室系统集成。 基于芯片和微流控的组合装置以及光学微谐振器在相当小的体积中提供快速、稳健、可重复和多重的生物检测能力。 我们详细介绍了迄今为止进行的研究，包括对各种寡核苷酸生物标志物以及脱氧核糖核酸 (DNA)、核糖核酸 (RNA) 和蛋白质的生物检测。 我们特别关注用于特定和选择性生物传感的化学表面修饰。
