Yoshioka T, Hirano R, Shioya T, Kako M
Technical Research Institute, Snow Brand Milk Products Co, Ltd, 1-2, Minamidai 1-Chome, Kawagoe, Saitama, Japan.
Biotechnol Bioeng. 1990 Jan 5;35(1):66-72. doi: 10.1002/bit.260350110.
Viable hybridoma cells were encapsulated. The capsules were formed in one step by placing a drop of cell suspension mixed with negatively charged carboxymethylcellulose (CMC) into a positively charged chitosan solution through the interpolymeric ionic interaction between two oppositely charged polymers. These capsules were found to have a mean diameter of about 1. 5 mm and wall thickness of 3 microm. The cells grew in the capsules using supplemented DMEM/F12 (four kinds of growth factor). The maximum cell density in encapsulating cell culture reached 1 x 10(7) cells/ml, 10 times higher than that obtained in the free cell culture. The maximum monoclonal antibody concentration in the free cell culture was 15 microg/mL, but that in the capsule was 45 microg/mL The antibody produced by the cell was concentrated about four times higher inside than outside of the capsules.
有活力的杂交瘤细胞被包封起来。通过将一滴与带负电荷的羧甲基纤维素（CMC）混合的细胞悬液，经由两种带相反电荷聚合物之间的聚合物间离子相互作用，滴入带正电荷的壳聚糖溶液中，一步形成胶囊。发现这些胶囊的平均直径约为1.5毫米，壁厚为3微米。细胞在添加了DMEM/F12（四种生长因子）的情况下在胶囊中生长。包封细胞培养中的最大细胞密度达到1×10⁷个细胞/毫升，比游离细胞培养中获得的密度高10倍。游离细胞培养中的最大单克隆抗体浓度为15微克/毫升，但胶囊中的为45微克/毫升。细胞产生的抗体在胶囊内部的浓度比外部高约四倍。
