Westwood A
Department of Paediatric Biochemistry, Royal Hospital for Sick Children, Edinburgh, UK.
Ann Clin Biochem. 1991 Mar;28 ( Pt 2):119-30. doi: 10.1177/000456329102800202.
The most widely used methods for the assay of total bilirubin in serum are based on a coupling reaction with various diazo dyes in the presence of an accelerating agent. The 'direct' reaction, without accelerator, gives a good estimate of the conjugated and protein bound (delta) species of bilirubin only if carefully standardized reaction conditions are used. Enzymatic methods based on bilirubin oxidase appear to give similar results, for both total and direct bilirubin, and through difficult to calibrate, spectrophotometric methods can also give reliable results for total bilirubin at the higher concentrations seen in neonates. More sophisticated HPLC or dry-slide techniques are required for the specific assay of the various conjugated and protein-bound species of bilirubin in serum.
血清总胆红素测定最常用的方法是基于在加速剂存在下与各种重氮染料的偶联反应。“直接”反应（无加速剂）只有在使用精心标准化的反应条件时，才能很好地估计结合胆红素和与蛋白质结合的（δ）胆红素种类。基于胆红素氧化酶的酶法似乎对总胆红素和直接胆红素都能给出类似的结果，尽管分光光度法难以校准，但对于新生儿中所见的较高浓度的总胆红素也能给出可靠的结果。血清中各种结合胆红素和与蛋白质结合的胆红素种类的特异性测定需要更复杂的高效液相色谱法或干片技术。
