基于聚多巴胺负载的金纳米簇膜用于游离胆红素的荧光传感

A Gold Nanoclusters Film Supported on Polydopamine for Fluorescent Sensing of Free Bilirubin.

机构信息

School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, China.

Guangxi Key Laboratory of Automatic Detection Technology and Instruments, Guilin University of Electronic Technology, Guilin 541004, China.

出版信息

Sensors (Basel). 2019 Apr 10;19(7):1726. doi: 10.3390/s19071726.

Serum bilirubin is an important biomarker for the diagnosis of various types of liver diseases and blood disorders. A polydopamine/gold nanoclusters composite film was fabricated for the fluorescent sensing of free bilirubin. Bovine serum albumin (BSA)-stabilized gold nanoclusters (AuNCs) were used as probes for biorecognition. The polydopamine film was utilized as an adhesion layer for immobilization of AuNCs. When the composite film was exposed to free bilirubin, due to the complex that was formed between BSA and free bilirubin, the fluorescence intensity of the composite film was gradually weakened as the bilirubin concentration increased. The fluorescence quenching ratio (F₀/F) was linearly proportional to free bilirubin over the concentration range of 0.8~50 μmol/L with a limit of detection of 0.61 ± 0.12 μmol/L (S/N = 3). The response was quick, the film was recyclable, and common ingredients in human serum did not interfere with the detection of free bilirubin.

血清胆红素是诊断各种类型肝脏疾病和血液疾病的重要生物标志物。本文制备了一种聚多巴胺/金纳米簇复合材料薄膜，用于游离胆红素的荧光传感。牛血清白蛋白（BSA）稳定的金纳米簇（AuNCs）被用作生物识别的探针。聚多巴胺薄膜被用作固定 AuNCs 的附着层。当复合膜暴露于游离胆红素时，由于 BSA 和游离胆红素之间形成的复合物，随着胆红素浓度的增加，复合膜的荧光强度逐渐减弱。荧光猝灭比（F₀/F）与游离胆红素在 0.8~50 μmol/L 的浓度范围内呈线性关系，检出限为 0.61 ± 0.12 μmol/L（S/N = 3）。该响应快速，薄膜可重复使用，且人血清中的常见成分不会干扰游离胆红素的检测。