Wu Shaobo, O'Leary Stephen J B, Gleddie Steve, Eudes François, Laroche André, Robert Laurian S
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, KW Neatby Bldg, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada.
Plant Cell Rep. 2008 Sep;27(9):1441-9. doi: 10.1007/s00299-008-0572-3. Epub 2008 Jul 1.
A novel anther-specific chalcone synthase-like gene, TaCHSL1, was isolated and characterized. The TaCHSL1 transcript was detected only within the tapetum during the "free" and early vacuolated microspore stages in both wheat and triticale. Sequence analysis indicated that the 41.8 kDa TaCHSL1 deduced protein belongs to a small distinct group of type III polyketide synthases that includes angiosperm and gymnosperm orthologs shown to be anther-specific. TaCHSL1 sequence characteristics and conservation, as well as its restricted expression pattern, point to a distinct and important biochemical role in developing anthers.
分离并鉴定了一个新的花药特异性查尔酮合酶样基因TaCHSL1。在小麦和小黑麦的“游离”和早期液泡化小孢子阶段，仅在绒毡层中检测到TaCHSL1转录本。序列分析表明，推导的41.8 kDa TaCHSL1蛋白属于III型聚酮合酶的一个小的独特亚组，该亚组包括被子植物和裸子植物中显示为花药特异性的直系同源物。TaCHSL1的序列特征和保守性，以及其受限的表达模式，表明它在花药发育中具有独特且重要的生化作用。
