胰岛素在3T3-L1前脂肪细胞向脂肪细胞分化过程中诱导酰基辅酶A结合蛋白及其mRNA的产生。
Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation.
作者信息
Hansen H O, Andreasen P H, Mandrup S, Kristiansen K, Knudsen J
机构信息
Institute of Biochemistry, University of Odense, Denmark.
出版信息
Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):341-4. doi: 10.1042/bj2770341.
Abstract
The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.
摘要
在3T3-L1细胞生长和胰岛素诱导分化过程中，研究了酰基辅酶A结合蛋白（ACBP）及其mRNA的诱导情况。在胰岛素刺激下，3T3-L1细胞从前脂肪细胞转变为完全成熟的脂肪细胞的过程中，ACBP相对于总可溶性蛋白和DNA的水平均有所增加。通过[1-14C]乙酸掺入法测定的脂肪生成总速率也出现了类似增加。相对于总RNA，ACBP mRNA也有类似的增加。因此，这些结果表明ACBP在脂肪生成过程中发挥着特定作用。然而，由于脂肪生成的增加先于ACBP的增加，这个作用可能是间接的。本文讨论了这一发现的意义。
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