5-甲基巯基-2'-脱氧尿苷对单纯疱疹病毒1型在两种细胞系统中复制的差异影响。
Differential effects of 5-methylmercapto-2'-deoxyuridine on the replication of herpes simplex virus type 1 in two cell systems.
作者信息
Hardi R, Hughes R G, Ho Y K, Chadha K C, Bardos T J
出版信息
Antimicrob Agents Chemother. 1976 Oct;10(4):682-6. doi: 10.1128/AAC.10.4.682.
5-Methylmercapto-2'-deoxyuridine (MeMUdR), a structural analogue of thymidine (TdR), inhibits herpes simplex virus type 1 production in mouse L (Lb) cells at concentrations that are not inhibitory to viral growth in monkey kidney (CV-1) cells. It is moderately toxic to Lb cells but not to CV-1 cells at a concentration that causes 95% inhibition of viral replication in Lb cells. MeMUdR is incorporated into cellular and viral deoxyribonucleic acid (DNA) in both systems, but to a significantly higher level (compared with thymidine) in Lb cells. These results indicate that MeMUdR is a substrate for enzymes leading to DNA synthesis and suggest that the biological function of herpes simplex virus type 1 DNA is impaired only when the incorporation of MeMUdR into the DNA reaches a relatively high level.
5-甲基巯基-2'-脱氧尿苷（MeMUdR）是胸腺嘧啶核苷（TdR）的结构类似物，在不抑制猴肾（CV-1）细胞中病毒生长的浓度下，可抑制小鼠L（Lb）细胞中单纯疱疹病毒1型的产生。在导致Lb细胞中病毒复制95%抑制的浓度下，它对Lb细胞有中度毒性，但对CV-1细胞无毒性。MeMUdR在两个系统中都被掺入细胞和病毒脱氧核糖核酸（DNA）中，但在Lb细胞中的掺入水平（与胸腺嘧啶核苷相比）显著更高。这些结果表明，MeMUdR是导致DNA合成的酶的底物，并表明只有当MeMUdR掺入DNA达到相对较高水平时，单纯疱疹病毒1型DNA的生物学功能才会受损。
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