Department of Feto-Maternal Medicine, Pomeranian Medical University, 1 Unii Lubelskiej Street, 71-252 Szczecin, Poland.
Eur J Obstet Gynecol Reprod Biol. 2009 Oct;146(2):165-8. doi: 10.1016/j.ejogrb.2009.06.016. Epub 2009 Jul 25.
This research was designed to analyze the presence of fetal female DNA, expressed in copy number, in the plasma of the pregnant woman with preeclampsia-complicated pregnancy.
Twenty-four pregnant women with female fetuses identified by means of ultrasound scanning were enrolled in this pilot study. The study group consisted of 12 pregnant women with symptoms of preeclampsia, with 12 healthy women, matched for gestational age, as controls.
Mean DNA number of genomic equivalents per reaction in the group was 201 geq/PCR (from 44.9 to 375) and increased over time after onset of PE, which was the reason for pregnancy termination. In the group of women with preeclampsia, a notably higher DNA copy number in comparison to the control group was noted (p=0.0003 U Mann-Whitney test).
The pilot study presented in this work confirms that also in the case of preeclampsia-complicated pregnancy with female fetuses it is possible to implement the method of fetal DNA quantification. Use of the presented methods confirms that in severe preeclampsia-complicated pregnancies an increase of the number of DNA genomic equivalents per reaction in comparison to the control group is observed. Due to the small study group further research on the described issue is vital, but this study proves that it is also feasible among women carrying female fetuses.
本研究旨在分析患有妊娠合并子痫前期孕妇血浆中胎儿女性 DNA 的存在情况，以拷贝数形式表现。
本研究纳入了 24 名通过超声扫描确认怀有女胎的孕妇。研究组由 12 名患有子痫前期症状的孕妇组成，对照组为 12 名年龄相匹配、无合并症的健康孕妇。
研究组中每个反应的平均基因组当量 DNA 数为 201 geq/PCR（范围为 44.9 至 375），并在子痫前期发作后随时间增加，这也是终止妊娠的原因。与对照组相比，子痫前期组的 DNA 拷贝数明显更高（p=0.0003 U 曼-惠特尼检验）。
本研究工作中的初步研究证实，即使是患有女性胎儿妊娠合并子痫前期的孕妇，也可以实施胎儿 DNA 定量的方法。所使用的方法证实，与对照组相比，严重妊娠合并子痫前期孕妇的每个反应的 DNA 基因组当量数增加。由于研究组规模较小，需要进一步研究该问题，但本研究证明，对于携带女胎的孕妇，这种方法也是可行的。
