Kulkarni R R, Parreira V R, Sharif S, Prescott J F
Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Vaccine. 2008 Aug 5;26(33):4194-203. doi: 10.1016/j.vaccine.2008.05.079. Epub 2008 Jun 17.
Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding three C. perfringens proteins: fructose-biphosphate-aldolase (FBA), pyruvate:ferredoxin-oxidoreductase (PFOR) and hypothetical protein (HP), were cloned into an avirulent Salmonella enterica sv. typhimurium vaccine vector. Broiler chickens immunized orally with recombinant Salmonella expressing FBA or HP proteins were significantly protected against NE challenge. Immunized birds developed serum and mucosal antibodies to both clostridial and Salmonella antigens. This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector.
肉鸡坏死性肠炎（NE）由产气荚膜梭菌引起，但目前尚无有效的疫苗。我们之前的研究表明，某些产气荚膜梭菌分泌蛋白经肌肉注射给药后可保护鸡免受实验性感染。在本研究中，编码三种产气荚膜梭菌蛋白的基因：果糖二磷酸醛缩酶（FBA）、丙酮酸：铁氧化还原蛋白氧化还原酶（PFOR）和假定蛋白（HP），被克隆到无毒的肠炎沙门氏菌鼠伤寒血清型疫苗载体中。口服表达FBA或HP蛋白的重组沙门氏菌免疫的肉鸡对NE攻击具有显著的保护作用。免疫鸡产生了针对梭菌和沙门氏菌抗原的血清抗体和粘膜抗体。本研究表明，两种产气荚膜梭菌抗原通过减毒沙门氏菌疫苗载体对肉鸡NE具有口服免疫能力。
