Pino M, Stingelin N, Tanner K E
Department of Mechanical Engineering, University of Glasgow, Glasgow G12 8QQ, UK.
Acta Biomater. 2008 Nov;4(6):1827-36. doi: 10.1016/j.actbio.2008.05.004. Epub 2008 May 23.
The skirt of an artificial cornea must integrate the implant to the host sclera, a major failure of present devices. Thus, it is highly desirable to encourage the metabolic activity of the cornea by using more bioactive, flexible skirt materials. Here we describe attempts to increase the bioactivity of polyether ether ketone (PEEK), high-density polyethylene (HDPE) and ultra-high molecular weight polyethylene (UHMWPE) films. The effectiveness of different strength NaOH pre-treatments to initiate apatite deposition on PEEK, HDPE and UHMWPE is investigated. We find that exposure of PEEK, HDPE and UHMWPE films to NaOH solutions induces the formation of potential nuclei for apatite (calcium phosphate), from which the growth of an apatite coating is stimulated when subsequently immersing the polymer films in 1.5 strength Simulated Body Fluid (SBF). As immersion time in SBF increases, further nucleation and growth produces a thicker and more compact apatite coating that can be expected to be highly bioactive. Interestingly, the apatite growth is found to also be dependent on both the concentration of NaOH solution and the structure of the polymer surface.
人工角膜的裙边必须将植入物与宿主巩膜整合在一起，而这正是目前设备的一个主要缺陷。因此，非常需要通过使用更具生物活性、柔韧性更好的裙边材料来促进角膜的代谢活性。在此，我们描述了提高聚醚醚酮（PEEK）、高密度聚乙烯（HDPE）和超高分子量聚乙烯（UHMWPE）薄膜生物活性的尝试。研究了不同强度的氢氧化钠预处理在PEEK、HDPE和UHMWPE上引发磷灰石沉积的效果。我们发现，将PEEK、HDPE和UHMWPE薄膜暴露于氢氧化钠溶液中会诱导形成磷灰石（磷酸钙）的潜在晶核，随后将聚合物薄膜浸入1.5强度的模拟体液（SBF）中时，会刺激磷灰石涂层的生长。随着在SBF中浸泡时间的增加，进一步的成核和生长会产生更厚、更致密的磷灰石涂层，预计该涂层具有很高的生物活性。有趣的是，发现磷灰石的生长还取决于氢氧化钠溶液的浓度和聚合物表面的结构。
