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通过静电纺丝一层胶原-羟基磷灰石复合纳米纤维对微纤维聚乳酸-羟基乙酸共聚物（PLGA）织物进行层压以用于骨组织工程。

Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering.

作者信息

Kwon Gi-Wan, Gupta Kailash Chandra, Jung Kyung-Hye, Kang Inn-Kyu

机构信息

Department of Polymer Science and Engineering, Kyungpook National University, Daegu, 702-701 South Korea.

Polymer Research Laboratory, Department of Chemistry, I. I. T. Roorkee, Roorkee, 247 667 India.

出版信息

Biomater Res. 2017 Jun 13;21:11. doi: 10.1186/s40824-017-0097-3. eCollection 2017.

DOI:10.1186/s40824-017-0097-3
PMID:28620549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5470256/
Abstract

BACKGROUND

To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen-hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1.5 mL/h and an applied voltage of 15 kV.

RESULTS

In comparison to pristine PLGA, dopamine-coated PLGA and collagen-hydroxyapatite composite nanofiber lamination has produced more wettable surfaces and surface wettability is found to higher with dopamine-coated PLGA fabrics then pristine PLGA. The SEM micrographs have clearly indicated that the lamination of polydopamine-coated PLGA fabric with collagen-hydroxyapatite composite nanofibers has shown increased adhesion of MC3T3E1 cells in comparison to pristine PLGA fabrics.

CONCLUSION

The results of these studies have clearly demonstrated that collagen-nHA composites fibers may be used to create bioactive 3D scaffolds using PLGA as an architectural support agent.

摘要

背景

为模拟通过蛋白质分泌实现的肌肉启发式细胞黏附，已成功在聚多巴胺（PDA）包覆的微纤维聚乳酸-乙醇酸共聚物（PLGA）织物上进行了胶原-羟基磷灰石纳米棒（nHA）复合纳米纤维的层压。通过以1.5 mL/h的流速和15 kV的施加电压静电纺丝含有L-谷氨酸接枝羟基磷灰石纳米棒（nHA-GA）的胶原溶液，在聚多巴胺包覆的微纤维PLGA织物上进行胶原-羟基磷灰石复合纳米纤维的层压。

结果

与原始PLGA相比，多巴胺包覆的PLGA和胶原-羟基磷灰石复合纳米纤维层压产生了更具润湿性的表面，并且发现多巴胺包覆的PLGA织物的表面润湿性高于原始PLGA。扫描电子显微镜图像清楚地表明，与原始PLGA织物相比，聚多巴胺包覆的PLGA织物与胶原-羟基磷灰石复合纳米纤维的层压显示MC3T3E1细胞的黏附增加。

结论

这些研究结果清楚地表明，胶原-nHA复合纤维可用于以PLGA作为结构支撑剂创建生物活性三维支架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/7a170e8f4be2/40824_2017_97_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/20fba8034386/40824_2017_97_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/5b57dc07d3b9/40824_2017_97_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/846bdb5044de/40824_2017_97_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/f8cacf73d35f/40824_2017_97_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/02670793e4ea/40824_2017_97_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/b7716ff89194/40824_2017_97_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/1712ba13b46c/40824_2017_97_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/5db219313200/40824_2017_97_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/d85203cd81c7/40824_2017_97_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/992f966f3df5/40824_2017_97_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/582495719570/40824_2017_97_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/7a170e8f4be2/40824_2017_97_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/20fba8034386/40824_2017_97_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/5b57dc07d3b9/40824_2017_97_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/846bdb5044de/40824_2017_97_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/f8cacf73d35f/40824_2017_97_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/02670793e4ea/40824_2017_97_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/b7716ff89194/40824_2017_97_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/1712ba13b46c/40824_2017_97_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/5db219313200/40824_2017_97_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/d85203cd81c7/40824_2017_97_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/992f966f3df5/40824_2017_97_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/582495719570/40824_2017_97_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f922/5470256/7a170e8f4be2/40824_2017_97_Fig12_HTML.jpg

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