Mitchell Claudia, Willenbring Holger
Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), Paris, France.
Nat Protoc. 2008;3(7):1167-70. doi: 10.1038/nprot.2008.80.
The ability of hepatocytes to enter the cell cycle and regenerate the liver after tissue loss provides an in vivo model to study the regulation of proliferation and organ regeneration. The extent of hepatocyte proliferation is directly proportional to the amount of resected liver tissue, and 2/3 partial hepatectomy (2/3 PH) leads to highly synchronized hepatocyte cell-cycle entry and progression. This surgical technique was first described in rats and requires modification for application in mice. Lack of standardization of 2/3 PH in mice has caused discrepancies in the results obtained in different laboratories. Here, we provide a protocol and a movie describing a straightforward surgical technique, which takes 15-20 min, to consistently remove two-thirds of the liver in mice. As this protocol is not associated with mortality and gives highly reproducible results, we hope that it will be widely used and serve to standardize 2/3 PH in mice.
肝细胞在组织损伤后进入细胞周期并再生肝脏的能力为研究增殖调控和器官再生提供了一个体内模型。肝细胞增殖的程度与切除的肝组织量直接成正比，三分之二肝部分切除术（2/3 PH）会导致肝细胞高度同步地进入细胞周期并进展。这种手术技术最早在大鼠中描述，应用于小鼠时需要进行改进。小鼠2/3 PH缺乏标准化导致不同实验室获得的结果存在差异。在此，我们提供了一份方案和一段视频，描述了一种简单的手术技术，该技术耗时15 - 20分钟，可始终如一地切除小鼠肝脏的三分之二。由于该方案不会导致小鼠死亡且结果具有高度可重复性，我们希望它能被广泛使用，并有助于规范小鼠的2/3 PH操作。
