Lorke Dietrich E, Ip Chi Wang, Schumacher Udo
Department of Anatomy, Faculty of Medicine and Health Sciences, UAE University, Box 17666, Al Ain, United Arab Emirates.
Histochem Cell Biol. 2008 Oct;130(4):693-7. doi: 10.1007/s00418-008-0463-2. Epub 2008 Jul 4.
To assess the in vivo influence of the systemic immune system upon microglia, six defined brain regions of adult SCID mice (n = 10) lacking functional T- and B-lymphocytes have been analyzed by NDPase histochemistry, morphometry and immunohistochemistry. Despite absence of neuropathology and lack of microglial activation, microglial numerical density was significantly increased in SCID mice. Elevation was most marked in the cerebellar granular layer (by 32.6%; 95% confidence interval: 9.9-58.7%), followed by the fimbria hippocampi and the molecular layer of hippocampal CA1/CA3 region. These data need to be taken into account when using SCID mice as a model for microglial reaction in immunodeficient mice.
为评估全身免疫系统对小胶质细胞的体内影响，我们采用NDP酶组织化学、形态计量学和免疫组织化学方法，对10只缺乏功能性T淋巴细胞和B淋巴细胞的成年SCID小鼠的六个特定脑区进行了分析。尽管没有神经病理学改变且小胶质细胞未被激活，但SCID小鼠的小胶质细胞数量密度显著增加。小脑颗粒层的增加最为明显（增加32.6%；95%置信区间：9.9 - 58.7%），其次是海马伞和海马CA1/CA3区的分子层。在将SCID小鼠用作免疫缺陷小鼠小胶质细胞反应模型时，需要考虑这些数据。
