人泪液中多种细胞因子分析：基于细胞计数微球法的优化流程
Multiple cytokine analysis in human tears: an optimized procedure for cytometric bead-based assay.
作者信息
LaFrance Martin W, Kehinde Lucy E, Fullard Roderick J
机构信息
School of Optometry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
出版信息
Curr Eye Res. 2008 Jul;33(7):525-44. doi: 10.1080/02713680802190085.
PURPOSE
To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears.
METHODS
Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated.
RESULTS
Luminextrade mark and Bio-Radtrade mark proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p < 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C.
CONCLUSION
Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.
目的
比较用于人眼泪中细胞因子基于细胞计数微球分析（CBA）的仪器和试剂。
方法
对来自五个供应商的两种CBA仪器和试剂盒进行平行比较。修改供应商方案以适应眼泪体积。评估精密度、回收率、稀释线性和稳定性。
结果
Luminex商标和Bio-Rad商标被证明对眼泪CBA最为适用。对于1 - 4微升的样本体积，观察到稀释线性具有良好的相关性（r > 0.80，p < 0.05）。样本在25摄氏度下30分钟内保持稳定。
结论
使用优化方案，通过CBA可以可靠地测量许多眼泪细胞因子。在与采集一致的时间段内浓度保持稳定。
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