Zhang Da-Lei, Mi Yu-Ling, Wang Kai-Ming, Zeng Wei-Dong, Zhang Cai-Qiao
College of Animal Sciences, Zhejiang University, Hangzhou, China.
J Zhejiang Univ Sci B. 2008 Jul;9(7):567-71. doi: 10.1631/jzus.B0820048.
The attenuating effect of daidzein (DAI) on oxidative toxicity induced by Aroclor 1254 (A1254) was investigated in mouse testicular cells. Cells were exposed to A1254 alone or with DAI. The oxidative damage was estimated by measuring malondialdehyde (MDA) formation, superoxide dismutase (SOD) activity and glutathione (GSH) content. Results show that A1254 induced a decrease of germ cell number, an elevation in thiobarbituric acid reactive substances (TBARS) but a decrease in SOD activity and GSH content. However, simultaneous supplementation with DAI decreased TBARS level and increased SOD activity and GSH content. Consequently, dietary DAI may restore the intracellular antioxidant system to attenuate the oxidative toxicity of A1254 in testicular cells.
在小鼠睾丸细胞中研究了大豆苷元（DAI）对艾氏剂1254（A1254）诱导的氧化毒性的衰减作用。细胞单独暴露于A1254或与DAI共同暴露。通过测量丙二醛（MDA）形成、超氧化物歧化酶（SOD）活性和谷胱甘肽（GSH）含量来评估氧化损伤。结果表明，A1254导致生殖细胞数量减少、硫代巴比妥酸反应性物质（TBARS）升高，但SOD活性和GSH含量降低。然而，同时补充DAI可降低TBARS水平并增加SOD活性和GSH含量。因此，饮食中的DAI可能恢复细胞内抗氧化系统，以减轻睾丸细胞中A1254的氧化毒性。
