Department of chemical Engineering, University of California at Berkeley, Berkeley, California 94720, USA.
Biotechnol Bioeng. 1991 Oct 5;38(7):733-41. doi: 10.1002/bit.260380707.
The steady-state metabolic parameters for a murine hybridoma cell line have been determined in continuous suspension culture over a wide range of dilution rates. Long-term adaption occurred over seven months in culture and resulted in lower glucose consumption rates, reduced lactate production, higher cell viability, and, consequently, growth rates more nearly matching the dilution rate. Antibody production rates decreased over the first two months and then remained stable for at least 75 days. The antibody production rate was not found to be growth associated. Steadystate amino acid uptake rates are presented for a wide range of growth rates.
已经在宽范围的稀释率下确定了在连续悬浮培养中的鼠杂交瘤细胞系的稳态代谢参数。在培养中经过七个月的长期适应，导致葡萄糖消耗率降低，乳酸生成减少，细胞活力增加，从而使生长速率更接近稀释率。抗体产生速率在前两个月下降，然后至少稳定 75 天。未发现抗体产生速率与生长相关。呈现了大范围生长速率下的稳态氨基酸摄取速率。
