Department of Chemical Engineering, University of California at Berkeley, Berkeley, California 94720, USA.
Biotechnol Bioeng. 1993 Jun 20;42(2):185-95. doi: 10.1002/bit.260420206.
The steady-state metabolic parameters for a hybridoma cell line have been determined in continuous suspension-perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at high perfusion rates and low cell bleed rates. At the low growth rates examined in this study, cellular metabolism shifted to become more oxidative, and as a result, the fraction of consumed substrate converted to inhibitory metabolic by-products was reduced. Specific antibody productivity was found to be non-growth associated.
已经在连续悬浮灌注培养中确定了杂交瘤细胞系的稳态代谢参数，在很宽的灌注率和细胞流出率范围内。在高灌注率和低细胞流出率下，可实现活细胞浓度和比生产率的显著提高。在本研究中检查的低生长速率下，细胞代谢转向更氧化，结果，消耗的基质转化为抑制代谢副产物的分数减少。发现特异性抗体生产率与生长无关。
