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2
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本文引用的文献

1
CO(2) in large-scale and high-density CHO cell perfusion culture.在大规模高密度 CHO 细胞灌注培养中 CO2 的作用。
Cytotechnology. 1996 Jan;22(1-3):65-78. doi: 10.1007/BF00353925.
2
The effect of dissolved oxygen on the metabolic profile of a murine hybridoma grown in serum-free medium in continuous culture.溶解氧对在无血清培养基中连续培养的小鼠杂交瘤细胞代谢谱的影响。
Biotechnol Bioeng. 1997 Apr 20;54(2):153-64. doi: 10.1002/(SICI)1097-0290(19970420)54:2<153::AID-BIT7>3.0.CO;2-K.
3
Material balance studies on animal cell metabolism using a stoichiometrically based reaction network.基于化学计量学反应网络的动物细胞代谢物质平衡研究。
Biotechnol Bioeng. 1996 Dec 5;52(5):579-90. doi: 10.1002/(SICI)1097-0290(19961205)52:5<579::AID-BIT5>3.0.CO;2-G.
4
Metabolic flux analysis of hybridoma cells in different culture media using mass balances.使用质量平衡法对不同培养基中的杂交瘤细胞进行代谢通量分析。
Biotechnol Bioeng. 1996 May 5;50(3):299-318. doi: 10.1002/(SICI)1097-0290(19960505)50:3<299::AID-BIT9>3.0.CO;2-B.
5
On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement.利用ATP平衡和氧化还原电位测量在线估计不同溶解氧水平下杂交瘤培养物中的活细胞。
Biotechnol Bioeng. 1996 Feb 5;49(3):277-83. doi: 10.1002/(SICI)1097-0290(19960205)49:3<277::AID-BIT5>3.0.CO;2-H.
6
A combined cell-cycle and metabolic model for the growth of hybridoma cells in steady-state continuous culture.用于杂交瘤细胞在稳态连续培养中生长的细胞周期与代谢联合模型。
Biotechnol Bioeng. 1995 Oct 5;48(1):49-65. doi: 10.1002/bit.260480109.
7
High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding.通过在线营养物补料实现杂交瘤细胞的高活细胞浓度补料分批培养。
Biotechnol Bioeng. 1995 Jun 20;46(6):579-87. doi: 10.1002/bit.260460611.
8
Determination of the respiration quotient in mammalian cell culture in bicarbonate buffered media.在碳酸氢盐缓冲培养基中测定哺乳动物细胞培养中的呼吸商。
Biotechnol Bioeng. 1995 Mar 20;45(6):524-35. doi: 10.1002/bit.260450610.
9
Intracellular flux analysis in hybridomas using mass balances and in vitro (13)C nmr.利用质量平衡和体外¹³C核磁共振技术对杂交瘤细胞进行细胞内通量分析。
Biotechnol Bioeng. 1995 Feb 20;45(4):292-303. doi: 10.1002/bit.260450403.
10
On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine.动物细胞培养中的在线气体分析：II. 氧摄取率估算方法及其在谷氨酰胺控制补料中的应用
Biotechnol Bioeng. 1995 Jan 5;45(1):54-62. doi: 10.1002/bit.260450108.

利用物质和能量平衡估算动物细胞培养的耗氧量和二氧化碳生成率。

Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.

机构信息

Biochemical Engineering Division, GBF - Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124, Braunschweig, Germany.

出版信息

Cytotechnology. 1999 May;29(3):159-66. doi: 10.1023/A:1008004618163.

DOI:10.1023/A:1008004618163
PMID:19003340
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3463396/
Abstract

Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and alanine) are found to be relatively constant for different hybridoma cell lines and may be used as regularities. The calculated rates of oxygen uptake and carbon dioxide evolution agree well with experimental values of several different cultures reported in the literature. This simple method gives the same results as calculated on the basis of a detailed metabolic reaction network.

摘要

利用物料平衡和还原程度平衡方程可以估算动物细胞培养物的耗氧率和二氧化碳释放率。对于不同的杂交瘤细胞系，细胞蛋白、单克隆抗体、生物量和除谷氨酰胺和丙氨酸外的氨基酸消耗的聚集成分、分子量和还原程度被发现是相对恒定的，可以作为规律。计算得到的耗氧率和二氧化碳释放率与文献中报道的几种不同培养物的实验值吻合较好。这种简单的方法与基于详细代谢反应网络计算得到的结果相同。