利用物质和能量平衡估算动物细胞培养的耗氧量和二氧化碳生成率。
Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.
机构信息
Biochemical Engineering Division, GBF - Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124, Braunschweig, Germany.
出版信息
Cytotechnology. 1999 May;29(3):159-66. doi: 10.1023/A:1008004618163.
Abstract
Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and alanine) are found to be relatively constant for different hybridoma cell lines and may be used as regularities. The calculated rates of oxygen uptake and carbon dioxide evolution agree well with experimental values of several different cultures reported in the literature. This simple method gives the same results as calculated on the basis of a detailed metabolic reaction network.
摘要
利用物料平衡和还原程度平衡方程可以估算动物细胞培养物的耗氧率和二氧化碳释放率。对于不同的杂交瘤细胞系，细胞蛋白、单克隆抗体、生物量和除谷氨酰胺和丙氨酸外的氨基酸消耗的聚集成分、分子量和还原程度被发现是相对恒定的，可以作为规律。计算得到的耗氧率和二氧化碳释放率与文献中报道的几种不同培养物的实验值吻合较好。这种简单的方法与基于详细代谢反应网络计算得到的结果相同。
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