Lee G M, Kim S J, Palsson B O
Department of Biotechnology, Korea Advanced Institute of Science and Technology, Taejun.
Cytotechnology. 1994;16(1):1-15. doi: 10.1007/BF00761774.
In order to determine whether the enhanced specific antibody productivity (qMAb) of calcium alginate-entrapped hybridoma is cell line-specific, calcium alginate-entrapped hybridomas (4A2 and DB9G8) were cultivated under the condition where we had previously observed significantly enhanced qMAb of calcium alginate-entrapped S3H5/gamma 2bA2 hybridoma. Unlike S3H5/gamma 2bA2 hybridoma, neither 4A2 nor DB9G8 hybridomas showed persistently enhanced qMAb when they were entrapped in calcium alginate beads. The enhanced qMAb of entrapped 4A2 and DB9G8 hybridomas, which was 2-3 times higher than the qMAb of free-suspended cells in a control experiment, was observed only during the early stage of the culture. During the early stage of the culture, the viable cell concentration decreased probably due to cell damage during the entrapment process. As cell growth resumed, the qMAb decreased to the similar level of qMAb of free-suspended cells within 5-7 days. Thus, we conclude that the enhanced qMAb of calcium alginate-entrapped hybridomas is cell line-specific.
为了确定海藻酸钙包封的杂交瘤细胞增强的特异性抗体生产率（qMAb）是否具有细胞系特异性，将海藻酸钙包封的杂交瘤细胞（4A2和DB9G8）在我们之前观察到海藻酸钙包封的S3H5/γ2bA2杂交瘤细胞qMAb显著增强的条件下进行培养。与S3H5/γ2bA2杂交瘤细胞不同，当4A2和DB9G8杂交瘤细胞被包封在海藻酸钙珠中时，它们均未表现出持续增强的qMAb。在对照实验中，包封的4A2和DB9G8杂交瘤细胞的qMAb增强，比游离悬浮细胞的qMAb高2至3倍，这仅在培养早期观察到。在培养早期，活细胞浓度可能由于包封过程中的细胞损伤而降低。随着细胞生长恢复，qMAb在5至7天内降至与游离悬浮细胞qMAb相似的水平。因此，我们得出结论，海藻酸钙包封的杂交瘤细胞增强的qMAb具有细胞系特异性。
