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Species-specific variation in efficacy of yeast genomic DNA isolation techniques assessed using amplified fragment length polymorphism.

作者信息

Fuller Linda J, MacKenzie Donald A, Roberts Ian N

机构信息

National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.

出版信息

Anal Biochem. 2008 Oct 1;381(1):154-6. doi: 10.1016/j.ab.2008.06.021. Epub 2008 Jun 18.

DOI:10.1016/j.ab.2008.06.021
PMID:18601896
Abstract

Methods for isolating genomic DNA from yeasts are optimized for strains of Saccharomyces cerevisiae. The DNeasy tissue kit proved to be effective with 65 additional yeast species, providing 0.1 to 4.7 microg DNA/ml culture with sufficient purity to give reproducible amplified fragment length polymorphism (AFLP) profiles, but was unsuccessful with 13 other species. Two alternative yeast DNA purification kits, MasterPure and Y-DER, were effective with 6 of these and 2 additional species, leaving only 9 species that remained recalcitrant to yielding sufficient amounts of DNA with the required purity.

摘要

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