Breen Danna M, Sanli Toran, Giacca Adria, Tsiani Evangelia
Faculty of Applied Health Sciences, Brock University, St. Catharines, Ont., Canada L2S 3A1.
Biochem Biophys Res Commun. 2008 Sep 12;374(1):117-22. doi: 10.1016/j.bbrc.2008.06.104. Epub 2008 Jul 2.
Although recent studies in vitro and in vivo indicate that the polyphenol resveratrol (RSV) has anti-diabetic properties, the exact mechanisms involved are not known. In the present study, we examined the effects of RSV and the mechanism of regulation of glucose uptake in skeletal muscle cells. In L6 myotubes RSV (100 microM) induced maximum stimulation of glucose (2DG) uptake (201+/-8.90% of control, p<0.001), an effect that was similar to insulin action. RSV-stimulated glucose uptake was abolished by AMPK inhibition. In the presence of the sirtuin inhibitor nicotinamide, RSV-stimulated 2DG uptake and AMPK phosphorylation were abolished. RSV did not stimulate significant translocation of GLUT4 or GLUT1 transporters. However, treatment with indinavir, a GLUT4 specific inhibitor, blocked RSV-stimulated glucose uptake. We propose that RSV elevates glucose uptake in muscle cells through a mechanism that involves sirtuins and AMPK and possibly stimulation of GLUT4 transporter intrinsic activity.
尽管最近的体外和体内研究表明，多酚白藜芦醇（RSV）具有抗糖尿病特性，但其确切机制尚不清楚。在本研究中，我们研究了RSV对骨骼肌细胞葡萄糖摄取的影响及其调节机制。在L6肌管中，RSV（100微摩尔）诱导葡萄糖（2-脱氧葡萄糖）摄取的最大刺激（为对照的201±8.90%，p<0.001），这一效应与胰岛素作用相似。RSV刺激的葡萄糖摄取被AMPK抑制所消除。在存在sirtuin抑制剂烟酰胺的情况下，RSV刺激的2-脱氧葡萄糖摄取和AMPK磷酸化被消除。RSV未刺激GLUT4或GLUT1转运蛋白的显著转位。然而，用GLUT4特异性抑制剂茚地那韦处理可阻断RSV刺激的葡萄糖摄取。我们认为，RSV通过一种涉及sirtuins和AMPK的机制，可能还有对GLUT4转运蛋白内在活性的刺激，来提高肌肉细胞中的葡萄糖摄取。
