睾酮和6 - N，2'- O - 二丁酰腺苷3':5'-环磷酸刺激大鼠精囊中的蛋白质和溶酶体酶分泌。
Testosterone and 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate stimulate protein and lysosomal enzyme secretion in rat seminal vesicle.
作者信息
Koenig H, Lu C Y, Bakay R
出版信息
Biochem J. 1976 Sep 15;158(3):543-7. doi: 10.1042/bj1580543.
Rat seminal-vesicle secretion was studied in vitro in a slice-incubation system. Seminal-vesicle slices were preincubated with 32Pi for 15 min, rinsed, and incubated in an isotope-free 'chase' medium for up to 4h. Gland slices spontaneously discharged protein, three lysosomal hydrolases and trichloroacetic acid-insoluble 32P into the medium in a time- and temperature-dependent manner. Testosterone (10 muM) and dibutyryl cyclic AMP (1 mM) stimulated the discharge of protein, acid hydrolases and trichloroacetic acid-insoluble 32P, and also stimulated the incorporation of 32Pi into trichloroacetic acid-insoluble components. The acid phosphatase and beta-N-acetylhexosaminidase isoenzymes were separated by isoelectric focusing. These hydrolases were secreted into the medium as acidic isoenzymes, presumably contained within primary lysosomes, whereas they occurred largely as less acidic and basic isoenzymes in the glandular tissue.
在切片孵育系统中对大鼠精囊分泌物进行了体外研究。精囊切片先用³²P₁孵育15分钟，冲洗后，在不含同位素的“追踪”培养基中孵育长达4小时。腺体切片以时间和温度依赖性方式将蛋白质、三种溶酶体水解酶和三氯乙酸不溶性³²P释放到培养基中。睾酮（10 μM）和二丁酰环磷腺苷（1 mM）刺激蛋白质、酸性水解酶和三氯乙酸不溶性³²P的释放，也刺激³²P₁掺入三氯乙酸不溶性成分中。通过等电聚焦分离酸性磷酸酶和β-N-乙酰己糖胺酶同工酶。这些水解酶以酸性同工酶的形式分泌到培养基中，推测存在于初级溶酶体内，而它们在腺组织中主要以酸性较弱和碱性的同工酶形式存在。
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