一种用于双光子显微镜的时空补偿声光扫描器，可提供大视野。

A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view.

作者信息

Kremer Y, Léger J-F, Lapole R, Honnorat N, Candela Y, Dieudonné S, Bourdieu L

机构信息

Laboratoire de Neurobiologie, UMR CNRS et ENS 8544, Ecole Normale Supérieure, Département de Biologie, 46rue d'Ulm, 75005 Paris.

出版信息

Opt Express. 2008 Jul 7;16(14):10066-76. doi: 10.1364/oe.16.010066.

DOI:10.1364/oe.16.010066

PMID:18607414

Abstract

Acousto-optic deflectors (AOD) are promising ultrafast scanners for non-linear microscopy. Their use has been limited until now by their small scanning range and by the spatial and temporal dispersions of the laser beam going through the deflectors. We show that the use of AOD of large aperture (13mm) compared to standard deflectors allows accessing much larger field of view while minimizing spatio-temporal distortions. An acousto-optic modulator (AOM) placed at distance of the AOD is used to compensate spatial and temporal dispersions. Fine tuning of the AOM-AOD setup using a frequency-resolved optical gating (GRENOUILLE) allows elimination of pulse front tilt whereas spatial chirp is minimized thanks to the large aperture AOD.

摘要

声光偏转器（AOD）是用于非线性显微镜的很有前景的超快扫描仪。到目前为止，它们的使用受到扫描范围小以及穿过偏转器的激光束的空间和时间色散的限制。我们表明，与标准偏转器相比，使用大孔径（13毫米）的声光偏转器可以在将时空畸变降至最低的同时获得更大的视野。放置在声光偏转器一定距离处的声光调制器（AOM）用于补偿空间和时间色散。使用频率分辨光学门控（GRENOUILLE）对AOM-AOD装置进行微调，可以消除脉冲前沿倾斜，而由于大孔径声光偏转器，空间啁啾得以最小化。