Centre de Transfert en Biotechnologie Microbiologie, Département de Génie Biochimique et Alimentaire, URA-CNRS-544, Institut National des Sciences Appliquées, Toulouse Cedex, France.
Biotechnol Bioeng. 1993 Nov 20;42(10):1151-6. doi: 10.1002/bit.260421004.
In nitrogen-limited media, growth and fatty acid formation by the oleaginous yeast Rhodotorula glutinis, i.e., yield and fatty acid cell content, have been characterized regarding carbon and nitrogen availabilities. It was shown that the formation of fatty acid free biomass was limited by nitrogen availability, whereas the fatty acid production was directly dependent on the consumed C/N ratio. According to these observations, the fraction of substrate consumed for fatty acid synthesis was estimated by using a simple method based on the actual yields, i.e., the mass of carbon source strictly converted into fatty acids and fatty acid free biomass. From these results, relationships were established allowing to predict in a simple and performing manner the maximal attainable fatty acid cell content and yield from the available carbon and nitrogen. These relationships were validated by using experimental data obtained by various authors with different yeast strains, and the proposed method was compared to the energetic and mass balance method previously described.
在氮限制的培养基中，产油酵母 Rhodotorula glutinis 的生长和脂肪酸形成，即产率和脂肪酸细胞含量，已经根据碳和氮的可用性进行了描述。结果表明，脂肪酸无生物质的形成受到氮可用性的限制，而脂肪酸的产生则直接取决于消耗的 C/N 比。根据这些观察结果，通过使用一种基于实际产率的简单方法，即严格转化为脂肪酸和脂肪酸无生物质的碳源的质量，估计了用于脂肪酸合成的底物消耗部分。根据这些结果，建立了关系，允许以简单有效的方式从可用的碳和氮预测最大可达到的脂肪酸细胞含量和产率。通过使用不同酵母菌株的不同作者获得的实验数据验证了这些关系，并将提出的方法与以前描述的能量和质量平衡方法进行了比较。
